过氧亚硝酸盐与花生四烯酸。花生四烯酸环氧化物的鉴定。

Peroxynitrite and arachidonic acid. Identification of arachidonate epoxides.

作者信息

Balazy M

机构信息

Department of Pharmacology, New York Medical College, Valhalla 10595, USA.

出版信息

Pol J Pharmacol. 1994 Nov-Dec;46(6):593-600.

PMID:7620519

Abstract

Peroxynitrite is a novel substance capable of oxidation of arachidonic acid. Treatment of [1-14C]arachidonic acid with peroxynitrite at pH 7.4 resulted in formation of a complex mixture of radioactive products. The isolation of these compounds and structural analysis by liquid chromatography/mass spectrometry revealed formation of three epoxyeicosatrienoic acids: 8,9-EET, 11,12-EET and 14,15-EET. These epoxides can play a role in peroxynitrite induced oxidative modification of arachidonic acid in cells.

摘要

过氧亚硝酸盐是一种能够氧化花生四烯酸的新型物质。在pH 7.4条件下用过氧亚硝酸盐处理[1-14C]花生四烯酸，会产生放射性产物的复杂混合物。通过液相色谱/质谱法分离这些化合物并进行结构分析，结果显示形成了三种环氧二十碳三烯酸：8,9-环氧二十碳三烯酸、11,12-环氧二十碳三烯酸和14,15-环氧二十碳三烯酸。这些环氧化物可能在过氧亚硝酸盐诱导的细胞内花生四烯酸氧化修饰过程中发挥作用。