Babkov V V, Fedorova Z F
Klin Lab Diagn. 1995 Mar-Apr(2):40-2.
A method based on a lower sensitivity of S forms to colicines, in comparison with R forms, was used for the selection of S forms of bacteria from dissociated cultures of E. coli O.124:K72, S. sonnei, and S. flexneri. The cultures are inoculated in nutrient agar from the external segment of the zone of bacterial growth suppression by colicines D, E1, and V+m, where the content of S forms is relatively higher at the expense of death of R forms. The content of S forms in the resultant cultures of different strains was 1.7 to 31.1 times higher that in the initial cultures.
与R型相比，S型对大肠杆菌素的敏感性较低，基于这一特性的方法被用于从大肠杆菌O.124:K72、宋内志贺氏菌和福氏志贺氏菌的解离培养物中筛选细菌的S型。将培养物接种于营养琼脂上，接种部位来自于被大肠杆菌素D、E1和V+m抑制细菌生长区域的外部，在该区域，由于R型的死亡，S型的含量相对较高。不同菌株最终培养物中S型的含量比初始培养物中的高1.7至31.1倍。
