Yano T, Takahashi S, Ichikawa T
Division of Applied Food Research, National Institute of Health and Nutrition, Tokyo, Japan.
Res Commun Mol Pathol Pharmacol. 1995 Mar;87(3):367-70.
In the present study, we examined whether active oxygen formed in the process of 4-nitroquinoline 1-oxide (4NQO) reduction by DT-diaphorase could induce oxidative stress on the pulmonary nuclei. The rapid production of OH- radical-like species after the start of the 4NQO reduction was observed, and subsequent induction of nuclear lipid peroxidation occurred. In conjugation with this event, DNA damage estimated as DNA single strand breaks (DNA-SSB) increased in a time-dependent manner. The induction of this DNA damage was partially inhibited by mannitol or vitamin E treatment. These findings suggest that the active oxygen generated in the process of the 4NQO reduction can induce oxidative damage on the pulmonary nuclei.
