Yano T, Obata Y, Ishikawa G, Hagiwara K, Ichikawa T
Division of Applied Food Research, National Institute of Health & Nutrition, Tokyo, Japan.
Int J Vitam Nutr Res. 1994;64(3):181-4.
Oxidative stress may play a partial role in chemically induced tumorigenesis in mice. Herein, we investigated the preventive effect of vitamin E on 4-nitroquinoline 1-oxide (4NQO)-induced oxidative damage on pulmonary nuclei and lung tumorigenesis in mice. At 4 weeks after 4NQO injection, the levels of nuclear thiobarbituric acid substances (TBARS) and DNA single strand breaks (DNA-SSB) in the lungs of mice treated with 4NQO were significantly higher than those in the control mice. The 4NQO-induced oxidative stress on the nuclei and DNA-SSB were significantly inhibited by vitamin E treatment. The nuclear alpha-tocopherol level in the 4NQO-treated group was significantly lower than that in the control, but the plasma alpha-tocopherol level in the former was slightly lower than that in the latter. Vitamin E feeding compensated the decrease of the level in the nuclei and plasma. The feeding on excessive vitamin E for 23 weeks after 4NQO injection could partly reduce the lung tumor incidence as well as lung tumor multiplicity in mice. These findings suggest that vitamin E could partly suppress 4NQO-induced lung tumorigenesis in mice, probably through the inhibition of 4NQO-induced oxidative damage on the nuclei.
氧化应激可能在化学诱导的小鼠肿瘤发生过程中发挥部分作用。在此，我们研究了维生素E对4-硝基喹啉1-氧化物（4NQO）诱导的小鼠肺细胞核氧化损伤及肺肿瘤发生的预防作用。在注射4NQO后4周，4NQO处理的小鼠肺组织中核硫代巴比妥酸物质（TBARS）水平和DNA单链断裂（DNA-SSB）水平显著高于对照小鼠。维生素E处理可显著抑制4NQO诱导的细胞核氧化应激及DNA-SSB。4NQO处理组的细胞核α-生育酚水平显著低于对照组，但前者的血浆α-生育酚水平略低于后者。补充维生素E可弥补细胞核和血浆中该水平的降低。在注射4NQO后连续23周给予过量维生素E可部分降低小鼠的肺肿瘤发生率及肺肿瘤数量。这些发现表明，维生素E可能通过抑制4NQO诱导的细胞核氧化损伤，部分抑制4NQO诱导的小鼠肺肿瘤发生。
