Tamaoki J, Takemura H, Kondo M, Chiyotani A, Sakai A, Konno K
First Department of Medicine, Tokyo Women's Medical College, Japan.
Res Commun Mol Pathol Pharmacol. 1995 Apr;88(1):39-50.
We studied the effect of Saiboku-to (TJ-96), an anti-allergic herbal medicine, on transepithelial potential difference of rabbit trachea and possible involvement of nitric oxide (NO) generation in vivo. Perfusion of TJ-96 on the tracheal mucosal surface increased PD in a concentration-dependent manner, the maximal increase from the baseline level and the concentration of TJ-96 required to produce a half-maximal effect (EC50) being 8.1 +/- 1.4 mV (mean +/- SE, P < 0.001) and 47 micrograms/ml. This effect was abolished by pretreatment with the Na channel blocker amiloride. NG-nitro-L-arginine methylester (L-NAME) but not NG-nitro-D-arginine methylester (D-NAME) inhibited TJ-96-induced increase in PD, and this inhibition was selectively reversed by L-arginine. These results suggest that TJ-96 stimulates Na absorption by airway epithelial cells probably through NO generation.
我们研究了抗过敏草药柴胡汤（TJ - 96）对兔气管跨上皮电位差的影响以及体内一氧化氮（NO）生成可能的参与情况。在气管黏膜表面灌注TJ - 96以浓度依赖的方式增加了电位差，相对于基线水平的最大增加量以及产生半数最大效应所需的TJ - 96浓度（EC50）分别为8.1±1.4 mV（平均值±标准误，P < 0.001）和47微克/毫升。用钠通道阻滞剂氨氯吡咪预处理可消除这种效应。NG - 硝基 - L - 精氨酸甲酯（L - NAME）而非NG - 硝基 - D - 精氨酸甲酯（D - NAME）抑制了TJ - 96诱导的电位差增加，并且这种抑制作用可被L - 精氨酸选择性逆转。这些结果表明，TJ - 96可能通过生成NO刺激气道上皮细胞对钠的吸收。
