Ketter R, von Ballestrem C L, Lampel S, Seitz G, Zang K D, Romanakis K, Wullich B
Institute of Human Genetics, University of The Saarland, Homburg, Germany.
Cancer Genet Cytogenet. 1995 Jun;81(2):109-14. doi: 10.1016/0165-4608(94)00226-2.
Nine endometrial carcinomas were examined for numerical aberrations of the chromosomes 1,7, and X by fluorescence in situ hybridization using highly repetitive chromosome-specific probes. In addition, a combination of a centromeric and a telomeric chromosome 1 probe was applied to detect structural chromosome 1 aberrations. Chromosome aberrations were found in six tumors. In four of these, an imbalance between 1q12 and 1p36 was detected, indicating the presence of an extra 1p- chromosome. In regard to the chromosomes 7 and X, monosomies and trisomies were found. Intratumoral genetic heterogeneity in endometrial carcinomas was detectable by FISH and flow cytometry. In conclusion, our findings confirm that chromosome 1 is frequently involved in structural chromosome changes, indicating chromosome 1 to be of importance in the evolution of endometrial carcinoma.
使用高度重复的染色体特异性探针，通过荧光原位杂交技术对9例子宫内膜癌进行1号、7号和X染色体的数目畸变检测。此外，应用着丝粒和端粒1号染色体探针组合检测1号染色体的结构畸变。在6例肿瘤中发现了染色体畸变。其中4例检测到1q12和1p36之间的失衡，表明存在额外的1p-染色体。关于7号和X染色体，发现了单体和三体情况。通过荧光原位杂交技术和流式细胞术可检测到子宫内膜癌的肿瘤内遗传异质性。总之，我们的研究结果证实1号染色体经常参与染色体结构变化，表明1号染色体在子宫内膜癌的发生发展中具有重要作用。
