Muresu R, Cossu A, Scarpa A M, Volpi E V, Rocca P C, Pintus A, Tibiletti M G, Tanda F, Pirastu M, Massarelli G
Istituto di Genetica Molecolare del CNR, Alghero, Italy.
Cancer Genet Cytogenet. 1998 Nov;107(1):37-42. doi: 10.1016/s0165-4608(98)00059-4.
Retrospective analysis of chromosomal changes in endometrial carcinoma was performed by fluorescence in situ hybridization on free nuclei isolated from formalin-fixed paraffin-embedded tissue. We examined 23 archival samples for numerical aberrations of chromosomes 1 and 10 with the use of specific DNA probes for the pericentromeric and centromeric regions of these two chromosomes. Numerical aberrations of chromosomes 1 and 10 were detected in 39% of the case analyzed, and the frequency of trisomy 10 tended to increase as the histological grade worsened. Our findings confirm the association of cytogenetic anomalies involving chromosomes 1 and 10 with endometrial carcinoma, as reported by other studies, and suggest that changes in centromere 10 copy number may correlate with the degree of tumor differentiation.
通过对从福尔马林固定石蜡包埋组织中分离出的游离细胞核进行荧光原位杂交，对子宫内膜癌的染色体变化进行了回顾性分析。我们使用针对这两条染色体着丝粒周围和着丝粒区域的特异性DNA探针，检查了23份存档样本中1号和10号染色体的数目畸变情况。在所分析的病例中，39%检测到了1号和10号染色体的数目畸变，并且随着组织学分级的恶化，10号染色体三体的频率有增加趋势。我们的研究结果证实了其他研究报道的涉及1号和10号染色体的细胞遗传学异常与子宫内膜癌的关联，并表明10号着丝粒拷贝数的变化可能与肿瘤分化程度相关。
