Takemori N, Hirai K, Onodera R, Saito N
Third Department of Internal Medicine, Asahikawa Medical College, Japan.
Cell Tissue Res. 1995 Jul;281(1):69-76. doi: 10.1007/BF00307959.
Granules consisting of periodically arranged membranous lamellae and amorphous electron-opaque material, i.e., periodic lamellar granules, are present in human neutrophils. To date, no extensive ultrastructural studies have been carried out on these granules because of their infrequent presence in neutrophils. The bone marrow of 18 cases of chronic myeloproliferative disorders, including one case of chronic neutrophilic leukemia in which periodic lamellar granules were frequently seen in neutrophils, was investigated by electron microscopy. Periodic lamellar granules were seen in neutrophils in 12 of the 18 cases at varying frequencies. They were preferentially seen in immature neutrophils. The transverse profiles of these granules revealed concentric complete/incomplete rings or periodic parallel straight lines, i.e., various patterns of lamellar arrangement were present. Periodic lamellar granules were positive for myeloperoxidase and lysozyme at the electron-microscopic level. These results suggest that these granules represent a primary neutrophil granule subtype. However, their functional and pathologic significance remains unknown.
由周期性排列的膜性薄片和无定形电子致密物质组成的颗粒，即周期性层状颗粒，存在于人类中性粒细胞中。由于它们在中性粒细胞中出现的频率较低，迄今为止尚未对这些颗粒进行广泛的超微结构研究。对18例慢性骨髓增殖性疾病患者的骨髓进行了电子显微镜检查，其中包括1例慢性中性粒细胞白血病，该病例中性粒细胞中经常可见周期性层状颗粒。18例中有12例中性粒细胞中可见不同频率的周期性层状颗粒。它们在未成熟中性粒细胞中更常见。这些颗粒的横断面显示出同心的完整/不完整环或周期性平行直线，即存在各种层状排列模式。在电子显微镜水平上，周期性层状颗粒对髓过氧化物酶和溶菌酶呈阳性。这些结果表明这些颗粒代表一种主要的中性粒细胞颗粒亚型。然而，它们的功能和病理意义仍然未知。
