Cheng G, Chan A, Chun M, Wong L
Department of Pathology, University of Hong Kong, Queen Mary Hospital.
Clin Lab Haematol. 1995 Mar;17(1):81-4. doi: 10.1111/j.1365-2257.1995.tb00323.x.
The antiglobulin gel test (DiaMed) was compared with conventional IAT methods in pretransfusion antibody screening in a tertiary hospital in Hong Kong. Antibody titrations showed that the gel system was more sensitive than the tube IAT method in detecting anti-D, anti-S, anti-s, but slightly less sensitive with anti-E. The gel tests detected all clinically significant warm reacting allo-antibodies detected by conventional IAT methods except one case of weak anti-E. It detected a case of anti-C missed in conventional screening. The number of serological investigations due to false positive screens, non-specific antibodies and clinically insignificant cold antibodies (anti-P, and anti-Lewis) were reduced. The gel system was fast and easy to use. It would reduce delays and inconvenience caused to patients due to false positive screens and non-specific antibodies without compromising detection of clinically significant antibodies. However, of concern was the failure to detect several cases of anti-Le(a) and anti-P1 that were reactive at 37 degrees C by conventional IAT and one case of anti-H in a patient with Parabombay A.
在香港一家三级医院，将抗球蛋白凝胶试验（DiaMed）与传统抗人球蛋白试验（IAT）方法用于输血前抗体筛查进行比较。抗体滴定显示，凝胶系统在检测抗-D、抗-S、抗-s方面比试管IAT方法更敏感，但在检测抗-E时敏感性略低。凝胶试验检测出了传统IAT方法检测出的所有临床上有意义的温反应性同种抗体，但有一例弱抗-E除外。它还检测出了传统筛查漏检的一例抗-C。因假阳性筛查、非特异性抗体和临床上无意义的冷抗体（抗-P和抗-Lewis）导致的血清学检查次数减少。凝胶系统快速且易于使用。它将减少因假阳性筛查和非特异性抗体给患者造成的延误和不便，同时不影响对临床上有意义抗体的检测。然而，令人担忧的是，未能检测出几例通过传统IAT在37℃有反应的抗-Le(a)和抗-P1，以及一例孟买型A患者的抗-H。
