感染单核细胞增生李斯特菌后，具有偏向性可变区库的T细胞受体αβ + CD4- CD8- T细胞早期出现。

Early appearance of T cell receptor alpha beta + CD4- CD8- T cells with a skewed variable region repertoire after infection with Listeria monocytogenes.

作者信息

Matsuzaki G, Li X Y, Kadena T, Song F, Hiromatsu K, Yoshida H, Nomoto K

机构信息

Department of Immunology, Kyushu University, Fukuoka, Japan.

出版信息

Eur J Immunol. 1995 Jul;25(7):1985-91. doi: 10.1002/eji.1830250728.

DOI:10.1002/eji.1830250728

PMID:7621874

Abstract

We found that the number of T cell receptor (TCR) alpha beta + CD4- CD8- T cells increased in the peritoneal cavity on day 5 after an intraperitoneal infection with Listeria monocytogenes strain EGD together with TCR gamma delta + CD4- CD8- T cells. Thereafter, the TCR alpha beta + CD4- CD8- T cells decreased to a normal level by day 14. The TCR alpha beta + CD4- CD8- T cells showed an activated T cell phenotype (L-selectin CD44 +) and expressed CD45/B220 and interleukin-2 receptor beta, but did not express heat stable antigen, which is expressed by the immature CD4- CD8- thymocytes. Furthermore, 20-30% of the TCR alpha beta + CD4- CD8- T cells expressed the NK1.1 natural killer cell marker. Analysis of the TCR V region repertoire of the TCR alpha beta + CD4- CD8- T cells induced by L. monocytogenes infection showed that more than 80% of the TCR alpha beta + CD4- CD8- T cells expressed TCR V beta 8 detected by anti-TCR V beta 8.1 and 8.2 mAb, and a reverse transcription-polymerase chain reaction analysis of V alpha 14 relative to V alpha 11 expression revealed that the TCR alpha beta + CD4- CD8- T cells expressed a higher level of V alpha 14, which was reported to be preferentially expressed by TCR alpha beta + CD4- CD8- thymocytes rather than conventional CD4+ T cells. The TCR alpha beta + CD4- CD8-T cells showed a proliferative response to anti-TCR alpha beta mAb stimulation. In contrast, they showed no response to stimulation with either Listeria antigen or 65-kDa heat shock protein of Mycobacterium bovis, which do stimulate the Listeria-specific TCR alpha beta + CD4- CD8- T cells and the Listeria-induced TCR gamma delta + T cells, respectively. These results suggest that the TCR alpha beta + CD4- CD8- T cells may recognize a restricted set of self antigens induced by L. monocytogenes infection, and that they contribute to host protection at an early stage of infection.

摘要

我们发现，在用单核细胞增生李斯特菌EGD菌株腹腔感染后的第5天，腹腔内T细胞受体（TCR）αβ⁺CD4⁻CD8⁻T细胞的数量增加，同时TCRγδ⁺CD4⁻CD8⁻T细胞数量也增加。此后，到第14天TCRαβ⁺CD4⁻CD8⁻T细胞数量降至正常水平。TCRαβ⁺CD4⁻CD8⁻T细胞表现出活化的T细胞表型（L-选择素CD44⁺），并表达CD45/B220和白细胞介素-2受体β，但不表达未成熟CD4⁻CD8⁻胸腺细胞所表达的热稳定抗原。此外，20%-30%的TCRαβ⁺CD4⁻CD8⁻T细胞表达NK1.1自然杀伤细胞标志物。对由单核细胞增生李斯特菌感染诱导的TCRαβ⁺CD4⁻CD8⁻T细胞的TCR V区库分析表明，超过80%的TCRαβ⁺CD4⁻CD8⁻T细胞表达抗TCR Vβ8.1和8.2单克隆抗体所检测到的TCR Vβ8，并且相对于Vα11表达的Vα14的逆转录-聚合酶链反应分析显示，TCRαβ⁺CD4⁻CD8⁻T细胞表达更高水平的Vα14，据报道Vα14优先由TCRαβ⁺CD4⁻CD8⁻胸腺细胞而非传统CD4⁺T细胞表达。TCRαβ⁺CD4⁻CD8⁻T细胞对抗TCRαβ单克隆抗体刺激表现出增殖反应。相比之下，它们对单核细胞增生李斯特菌抗原或牛分枝杆菌65-kDa热休克蛋白的刺激均无反应，而这两种抗原分别可刺激李斯特菌特异性TCRαβ⁺CD4⁻CD8⁻T细胞和李斯特菌诱导的TCRγδ⁺T细胞。这些结果表明，TCRαβ⁺CD4⁻CD8⁻T细胞可能识别由单核细胞增生李斯特菌感染诱导的一组受限的自身抗原，并且它们在感染早期有助于宿主防御。