Penninger J M, Schilham M W, Timms E, Wallace V A, Mak T W
Amgen Institute, Princess Margaret Hospital, Toronto, Canada.
Eur J Immunol. 1995 Jul;25(7):2115-8. doi: 10.1002/eji.1830250748.
CD4-CD8- double-negative T cells constitute a lymphocyte subpopulation within the thymus and peripheral lymphatic organs that express a unique T cell receptor (TCR) repertoire and do not undergo negative selection. To test whether these cells develop as a distinct lineage or due to altered selection in the absence of CD4 and CD8 expression, we analyzed the TCR repertoire in mice lacking both CD4 and CD8 accessory molecules after homologous recombination (CD40/0CD80/0). We show that mature T cells of CD40/0CD80/0 mice express an unbiased diverse TCR V beta repertoire comparable to wild type mice. In addition, clonal deletion of mouse mammary tumor virus superantigen-reactive T cells did occur in CD40/0CD80/0 mice. These data show that the intrinsic lack of CD4 and CD8 expression has no effect on the mature TCR repertoire and that clonal deletion of superantigen-reactive cells is independent of CD4 and CD8 co-receptors.
CD4-CD8-双阴性T细胞构成胸腺和外周淋巴器官内的一个淋巴细胞亚群，该亚群表达独特的T细胞受体（TCR）库且不经历阴性选择。为了测试这些细胞是作为一个独特的谱系发育，还是由于在缺乏CD4和CD8表达的情况下选择改变而发育，我们分析了同源重组后缺乏CD4和CD8辅助分子的小鼠（CD40/0CD80/0）中的TCR库。我们发现，CD40/0CD80/0小鼠的成熟T细胞表达与野生型小鼠相当的无偏向性多样TCR Vβ库。此外，CD40/0CD80/0小鼠中确实发生了小鼠乳腺肿瘤病毒超抗原反应性T细胞的克隆性缺失。这些数据表明，内在缺乏CD4和CD8表达对成熟TCR库没有影响，并且超抗原反应性细胞的克隆性缺失独立于CD4和CD8共受体。
