建立一种具有鸟氨酸氨甲酰基转移酶活性且在精氨酸缺乏培养基中持续生长的大鼠肝癌细胞系。
Establishment of a rat hepatoma cell line which has ornithine carbamoyltransferase activity and grows continuously in arginine-deprived medium.
作者信息
Niwa A, Yamamoto K, Yasumura Y
出版信息
J Cell Physiol. 1979 Jan;98(1):177-84. doi: 10.1002/jcp.1040980119.
PMID:762194
Abstract
The epithelial cell line, H4-II-E derived from Reuber hepatoma H35 has no significant activity of ornithine carbamoyltransferase (OCT, EC 2.1.3.3) and is not able to grow in arginine-deprived medium. A multi-step selection procedure is described which selects from HR-II-E populations, cells with OCT activity which can grow in arginine-deficient, ornithine-supplemented media.
摘要
源自鲁伯氏肝癌H35的上皮细胞系H4-II-E没有明显的鸟氨酸氨甲酰基转移酶（OCT，EC 2.1.3.3）活性，并且不能在精氨酸缺乏的培养基中生长。本文描述了一种多步骤选择程序，该程序从H4-II-E细胞群体中筛选出具有OCT活性的细胞，这些细胞能够在精氨酸缺乏、添加鸟氨酸的培养基中生长。
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