Ahn T I, Jeon K W
J Cell Physiol. 1979 Jan;98(1):49-57. doi: 10.1002/jcp.1040980107.
A strain of nonsymbiotic A. proteus was infected with endosymbiotic bacteria isolated from another strain of amoeba which had become dependent on the symbionts after a few years of spontaneously established symbiosis. In the newly infected amoebae, the bacteria avoided digestion and multiplied at a faster rate than the hosts, reaching the maximum carrying number (about 42,000 per amoeba) in fewer than ten cell generations of the hosts. The experimentally infected amoebae were also examined under the electron microscope, and the development of bacteria-containing vesicles was followed. The results show that the infective bacteria that were initially harmful to host amoebae have become harmless and that they have changed in their mode of multiplication during the course of establishing a stable symbiosis with their hosts.
从另一株变形虫中分离出的内共生细菌感染了一株非共生的变形杆菌。这株变形虫在经过数年自发形成的共生关系后，已变得依赖这些共生体。在新感染的变形虫中，这些细菌避免了被消化，并且繁殖速度比宿主更快，在宿主不到十个细胞世代的时间内就达到了最大承载数量（每个变形虫约42,000个）。还在电子显微镜下检查了实验感染的变形虫，并追踪了含细菌囊泡的发育情况。结果表明，最初对宿主变形虫有害的感染性细菌已变得无害，并且在与宿主建立稳定共生关系的过程中，它们的繁殖方式发生了变化。
