Jeon K W, Jeon M S
J Cell Physiol. 1976 Oct;89(2):337-44. doi: 10.1002/jcp.1040890216.
A strain of large, free-living amoeba that became dependent on bacterial endosymbionts which had infected the amoebae initially as intracellular parasites, was studied by micrurgy and electron microscopy. The results show that the infected host cells require the presence of live endosymbionts for their survival.Thus, the nucleus of an infected amoeba can form a viable cell with the cytoplasm of a noninfected amoeba only when live endosymbionts are present. The endosymbiotic bacteria are not digested by the host amoebae and are not themselves used as nutritional supplement. While the host amoebae are dependent specifically on the endosymbionts, the latter can live inside amoebae of different strains, indicating that their dependence on the host cells is not yet strain specific.
研究人员通过显微手术和电子显微镜对一种大型自由生活变形虫菌株进行了研究，该菌株最初被细胞内寄生虫感染，之后变得依赖这些细菌内共生体。结果表明，受感染的宿主细胞需要活的内共生体才能存活。因此，只有当活的内共生体存在时，受感染变形虫的细胞核才能与未受感染变形虫的细胞质形成一个有活力的细胞。内共生细菌不会被宿主变形虫消化，也不会被用作营养补充。虽然宿主变形虫特别依赖内共生体，但后者可以生活在不同菌株的变形虫体内，这表明它们对宿主细胞的依赖尚未具有菌株特异性。
