deLapeyrière O, Naquet P, Planche J, Marchetto S, Rottapel R, Gambarelli D, Rosnet O, Birnbaum D
Laboratoire d'Oncologie Moléculaire, U.119 Inserm, Marseille, France.
Differentiation. 1995 Jun;58(5):351-9. doi: 10.1046/j.1432-0436.1995.5850351.x.
The Flt3 gene encodes a tyrosine kinase receptor highly related to the Kit and Fms gene products. We have studied the expression of Flt3 by using in situ hybridization of mouse tissue sections. The results show that Flt3 RNAs are present in certain regions of lymphohematopoietic organs, placenta and nervous system. Flt3 is expressed in the medullary area of fetal and newborn thymus, in the paracortical regions of lymph nodes and in the red pulp of spleen. In placenta, labyrinthine trophoblasts express Flt3. Finally, Flt3 RNAs are found in several regions of the brain and in cerebellar Purkinje cells. Western-blot analysis showed that the FLT3 protein is present in the tissues positive for Flt3 RNA expression. Our observations allow for a comparison with the distribution of the Kit gene and analysis of a possible redundancy between KIT and FLT3 receptors.
Flt3基因编码一种与Kit和Fms基因产物高度相关的酪氨酸激酶受体。我们通过对小鼠组织切片进行原位杂交来研究Flt3的表达。结果显示，Flt3 RNA存在于淋巴造血器官、胎盘和神经系统的某些区域。Flt3在胎儿和新生儿胸腺的髓质区域、淋巴结的副皮质区域以及脾脏的红髓中表达。在胎盘中，迷路滋养层细胞表达Flt3。最后，在大脑的几个区域以及小脑浦肯野细胞中发现了Flt3 RNA。蛋白质免疫印迹分析表明，FLT3蛋白存在于Flt3 RNA表达阳性的组织中。我们的观察结果有助于与Kit基因的分布进行比较，并分析KIT和FLT3受体之间可能存在的冗余情况。
