Ito A, Hirota S, Kitamura Y, Nomura S
Department of Pathology, Osaka University Medical School, Suita, Japan.
J Mol Neurosci. 1993 Winter;4(4):235-43. doi: 10.1007/BF02821555.
The expression and localization of flt3 mRNA were investigated using Northern blotting and in situ hybridization during the developmental process of the mouse brain. By Northern blotting, the expression of the flt3 gene was not detected in embryonic, neonatal, or early postnatal brains, but was markedly increased with age. In adult mice, flt3 was abundantly expressed in the cerebellum, moderately in the pons, and faintly in the thalamus and the cerebral cortex. By in situ hybridization, some neurons that expressed flt3 formed synaptic connections with each other. The present findings suggest that the flt3 gene expression is related to the cell type and the developmental process.
在小鼠大脑发育过程中，采用Northern印迹法和原位杂交技术研究了flt3 mRNA的表达及定位。通过Northern印迹法检测发现，在胚胎期、新生期或出生后早期的大脑中未检测到flt3基因的表达，但随着年龄增长其表达显著增加。在成年小鼠中，flt3在小脑中大量表达，在脑桥中适度表达，在丘脑和大脑皮质中微弱表达。通过原位杂交发现，一些表达flt3的神经元彼此之间形成了突触连接。目前的研究结果表明，flt3基因表达与细胞类型和发育过程有关。
