Bae S C, Takahashi E, Zhang Y W, Ogawa E, Shigesada K, Namba Y, Satake M, Ito Y
Department of Viral Oncology, Kyoto University, Japan.
Gene. 1995 Jul 4;159(2):245-8. doi: 10.1016/0378-1119(95)00060-j.
PEBP2/CBF is a heterodimeric transcription factor composed of alpha and beta subunits. Previously, we reported two distinct mouse genes, PEBP2 alpha A and PEBP2 alpha B, which encode the alpha subunit. PEBP2 alpha B is the homologue of human AML1, encoding the acute myeloid leukemia 1 protein. AML1 and human PEBP2/CBF beta were detected independently at the breakpoints of two characteristic chromosome translocations observed frequently in two subtypes of acute myeloid leukemia. The PEBP2 alpha proteins contain a 128-amino-acid (aa) region highly homologous to the Drosophila melanogaster segmentation gene runt. The evolutionarily conserved region, named the Runt domain, harbors DNA-binding and heterodimerizing activities. In this study, we identified the third Runt-domain-encoding gene, PEBP2 alpha C, which maps to 1p36.11-p36.13 in the human chromosome and encodes a 415-aa protein. PEBP2 alpha C forms a heterodimer with PEBP2 beta, binds to the PEBP2 site and transactivates transcription, similar to PEBP2 alpha A and PEBP2 alpha B.
PEBP2/CBF是一种由α和β亚基组成的异二聚体转录因子。此前，我们报道了两个不同的小鼠基因，即PEBP2αA和PEBP2αB，它们编码α亚基。PEBP2αB是人类AML1的同源物，编码急性髓系白血病1蛋白。在急性髓系白血病的两个亚型中经常观察到的两种特征性染色体易位的断点处，分别独立检测到了AML1和人类PEBP2/CBFβ。PEBP2α蛋白含有一个与黑腹果蝇分节基因runt高度同源的128个氨基酸（aa）区域。这个进化上保守的区域，称为Runt结构域，具有DNA结合和异二聚化活性。在本研究中，我们鉴定出了第三个编码Runt结构域的基因PEBP2αC，它定位于人类染色体的1p36.11 - p36.13，编码一个415个氨基酸的蛋白质。与PEBP2αA和PEBP2αB类似，PEBP2αC与PEBP2β形成异二聚体，结合到PEBP2位点并反式激活转录。
