Koettnitz K, Wöhl T, Kappel B, Lottspeich F, Hauber J, Bevec D
SANDOZ Research Institute, Vienna, Austria.
Gene. 1995 Jul 4;159(2):283-4. doi: 10.1016/0378-1119(95)00136-t.
Using an oligodeoxynucleotide generated by rapid PCR amplification of 5'-cDNA ends (5'-RACE) as a detection probe, we have isolated a new genomic clone encoding the human eukaryotic initiation factor 5A (eIF-5A). Sequence analysis revealed that the eIF-5A coding region is identical to the corresponding cDNA but interrupted by three introns. In a plasmid shuffle experiment we show functional replacement of the essential homologous gene in Saccharomyces cerevisiae by this human eIF-5A.
利用通过5'-cDNA末端快速PCR扩增（5'-RACE）产生的寡脱氧核苷酸作为检测探针，我们分离出了一个编码人真核起始因子5A（eIF-5A）的新基因组克隆。序列分析表明，eIF-5A编码区与相应的cDNA相同，但被三个内含子中断。在质粒洗牌实验中，我们证明了该人eIF-5A可在功能上替代酿酒酵母中的必需同源基因。
