Pfisterer J, Kommoss F, Sauerbrei W, Rendl I, Kiechle M, Kleine W, Pfleiderer A
Department of Obstetrics and Gynecology, Albert-Ludwigs-University of Freiburg, Germany.
Gynecol Oncol. 1995 Aug;58(2):149-56. doi: 10.1006/gyno.1995.1202.
Both nuclear DNA content and S-phase fraction (SPF) can be helpful in predicting prognosis in certain malignancies. We investigated in a retrospective study the prognostic significance of nuclear DNA content and SPF as measured by flow cytometry of tumor specimens from 162 women with nonpretreated surgically staged FIGO stage I endometrial cancer using clearly defined inclusion criteria. A total of 139 (86%) cases were found to be diploid, whereas 23 (14%) were aneuploid. Ploidy showed a correlation with histologic grade, estrogen as well as progesterone receptor levels, and depth of myometrial infiltration. Univariate analysis of follow-up data showed an increased relative risk (RR) for recurrence-free survival (RFS) for grade 3 tumors (RR = 2.11, ns), for age (RR = 1.04, P = 0.023) as a continuous variable, and for SPF in diploid tumors (RR = 3.10, P = 0.035). In addition, univariate analysis of overall survival revealed similar results with a slightly increased relative risk for ploidy (RR = 1.52, ns). Multivariate analysis of RFS showed age as the only independent prognostic factor. Multivariate analysis of RFS for diploid tumors showed no independently significant factor; however, age as a continuous variable with a relative risk of 1.04 and SPF with a relative risk of 2.94 were of borderline significance. Our results suggest that abnormalities of the nuclear DNA content and SPF in this homogeneous group of patients are associated with clinical and morphological prognosticators; however, ploidy is no independent prognostic factor for RFS. For diploid tumors, SPF might be a possible independent prognostic factor.
核DNA含量和S期分数（SPF）在某些恶性肿瘤的预后预测中都可能有所帮助。我们进行了一项回顾性研究，采用明确界定的纳入标准，通过流式细胞术检测162例未经治疗、手术分期为国际妇产科联盟（FIGO）I期子宫内膜癌的女性肿瘤标本的核DNA含量和SPF，以研究其预后意义。共发现139例（86%）为二倍体，而23例（14%）为非整倍体。倍性与组织学分级、雌激素以及孕激素受体水平和肌层浸润深度相关。对随访数据的单因素分析显示，3级肿瘤无复发生存期（RFS）的相对风险（RR）增加（RR = 2.11，无统计学意义），年龄作为连续变量时RR增加（RR = 1.04，P = 0.023），二倍体肿瘤的SPF也增加（RR = 3.10，P = 0.035）。此外，总生存的单因素分析显示了类似结果，倍性的相对风险略有增加（RR = 1.52，无统计学意义）。RFS的多因素分析显示年龄是唯一的独立预后因素。二倍体肿瘤RFS的多因素分析未显示独立的显著因素；然而，作为连续变量的年龄相对风险为1.04，SPF相对风险为2.94具有临界显著性。我们的结果表明，在这一同质患者群体中，核DNA含量和SPF异常与临床和形态学预后指标相关；然而，倍性不是RFS的独立预后因素。对于二倍体肿瘤，SPF可能是一个潜在的独立预后因素。
