Fujii T, Tsukazaki K, Kiguchi K, Kubushiro K, Yajima M, Nozawa S
Department of Obstetrics and Gynecology, School of Medicine, Keio University, Tokyo, Japan.
Gynecol Oncol. 1995 Aug;58(2):210-5. doi: 10.1006/gyno.1995.1213.
HPV-16 is implicated in the development of progressive cervical neoplasia, and E6 and E7 proteins of this virus play important roles in its oncogenic activity. Reverse transcriptase-nested-polymerase chain reaction (RT-nested PCR) method was applied to detect the major transcript (E6I/E7) from E6/E7 ORFs of HPV-16 in the exfoliated cervical and vaginal cells from cervical neoplasia patients. The incidence of the E6I/E7 transcript was proportional to the class of cytologic diagnosis. Further analysis revealed that the incidence of the E6I/E7 transcript in Pap smears of class I or II was 5/28 (18%) patients who had previously been diagnosed as having cervical neoplasia but was 1/37 (3%) patients who had never been diagnosed (P < 0.05). These findings suggest that some patients followed up for cervical neoplasia were infected with transcriptionally active HPVs even when their Pap smears were negative. In addition, the E6I/E7 transcript could not be detected after surgical treatment in any of the cases that were able to be followed up. The E6*I/E7 transcript in the exfoliated cells may prove valuable for epidemiological studies of the pathogenesis of HPV infection.
人乳头瘤病毒16型（HPV - 16）与宫颈上皮内瘤变的进展有关，该病毒的E6和E7蛋白在其致癌活性中起重要作用。采用逆转录巢式聚合酶链反应（RT - 巢式PCR）方法检测宫颈上皮内瘤变患者宫颈和阴道脱落细胞中HPV - 16的E6/E7开放阅读框的主要转录本（E6I/E7）。E6I/E7转录本的发生率与细胞学诊断类别成正比。进一步分析显示，I级或II级巴氏涂片患者中，曾被诊断为宫颈上皮内瘤变的患者E6I/E7转录本的发生率为5/28（18%），而从未被诊断过的患者为1/37（3%）（P < 0.05）。这些发现表明，一些接受宫颈上皮内瘤变随访的患者即使巴氏涂片结果为阴性，也感染了转录活跃的人乳头瘤病毒。此外，在所有能够进行随访的病例中，手术后均未检测到E6I/E7转录本。脱落细胞中的E6*I/E7转录本可能对人乳头瘤病毒感染发病机制的流行病学研究具有重要价值。
