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1
Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology.通过逆转录聚合酶链反应检测人乳头瘤病毒16型早期基因转录与宫颈细胞学异常相关。
J Clin Microbiol. 1997 Jun;35(6):1560-4. doi: 10.1128/jcm.35.6.1560-1564.1997.
2
Comparison of human papillomavirus messenger RNA and DNA detection: a cross-sectional study of 4,136 women >30 years of age with a 2-year follow-up of high-grade squamous intraepithelial lesion.人乳头瘤病毒信使核糖核酸与脱氧核糖核酸检测的比较：一项针对4136名30岁以上女性的横断面研究，对高级别鳞状上皮内病变进行了为期两年的随访。
Cancer Epidemiol Biomarkers Prev. 2005 Feb;14(2):367-72. doi: 10.1158/1055-9965.EPI-04-0410.
3
Evaluation of human papillomavirus testing in primary screening for cervical abnormalities: comparison of sensitivity, specificity, and frequency of referral.人乳头瘤病毒检测用于子宫颈异常初步筛查的评估：敏感性、特异性及转诊频率的比较
JAMA. 2002 Oct 9;288(14):1749-57. doi: 10.1001/jama.288.14.1749.
4
DNA- versus RNA-based methods for human papillomavirus detection in cervical neoplasia.基于DNA与RNA的方法用于宫颈肿瘤中人类乳头瘤病毒的检测
Gynecol Oncol. 2005 Jun;97(3):908-15. doi: 10.1016/j.ygyno.2005.02.026. Epub 2005 Apr 15.
5
Human papillomavirus typing and viral gene expression analysis for the triage of women with abnormal results from papanicolaou test smears to colposcopy.对巴氏涂片检查结果异常的女性进行分流以进行阴道镜检查的人乳头瘤病毒分型及病毒基因表达分析。
Arch Pathol Lab Med. 2009 Oct;133(10):1577-86. doi: 10.5858/133.10.1577.
6
Clinical Value of Human Papillomavirus E6/E7 mRNA Detection in Screening for Cervical Cancer in Women Positive for Human Papillomavirus DNA or.人乳头瘤病毒E6/E7 mRNA检测在人乳头瘤病毒DNA阳性女性宫颈癌筛查中的临床价值
Clin Lab. 2018 Sep 1;64(9):1363-1371. doi: 10.7754/Clin.Lab.2018.180138.
7
Human papillomavirus 16 E6/E7 transcript and E2 gene status in patients with cervical neoplasia.宫颈肿瘤患者中人乳头瘤病毒16 E6/E7转录本及E2基因状态
Mol Diagn. 2004;8(1):57-64. doi: 10.1007/BF03260048.
8
The clinical value of digene hybrid capture HPV DNA testing in a referral-based population with abnormal pap smears.在基于转诊的巴氏涂片异常人群中，Digene杂交捕获HPV DNA检测的临床价值。
Eur J Gynaecol Oncol. 1998;19(3):203-8.
9
Detection of high-risk human papillomavirus E6 and E7 oncogene transcripts in cervical scrapes by nested RT-polymerase chain reaction.通过巢式逆转录聚合酶链反应检测宫颈刮片中高危型人乳头瘤病毒E6和E7癌基因转录物
J Med Virol. 2004 Sep;74(1):107-16. doi: 10.1002/jmv.20153.
10
T cell proliferative responses against human papillomavirus type 16 E7 oncoprotein are most prominent in cervical intraepithelial neoplasia patients with a persistent viral infection.针对人乳头瘤病毒16型E7癌蛋白的T细胞增殖反应在持续病毒感染的宫颈上皮内瘤变患者中最为显著。
J Gen Virol. 1996 Sep;77 ( Pt 9):2183-91. doi: 10.1099/0022-1317-77-9-2183.

引用本文的文献

1
Decoding the Genetic and Structural Features of HPV16 E5 Oncogene in Cervical Cancer Isolates from Pakistan: A Pilot Study.解码来自巴基斯坦宫颈癌分离株中HPV16 E5癌基因的遗传和结构特征：一项试点研究。
Iran Biomed J. 2023 Aug 23;27(6):388-96. doi: 10.61186/ibj.3884.
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Cytotoxic T-lymphocyte responses to human papillomavirus type 16 E5 and E7 proteins and HLA-A*0201-restricted T-cell peptides in cervical cancer patients.宫颈癌患者对人乳头瘤病毒16型E5和E7蛋白以及HLA - A*0201限制性T细胞肽的细胞毒性T淋巴细胞反应。
J Virol. 2007 Mar;81(6):2869-79. doi: 10.1128/JVI.02256-06. Epub 2007 Jan 3.
3
High-risk human papillomavirus type 16 E7 oncogene associates with Cdc25A over-expression in oral squamous cell carcinoma.高危型人乳头瘤病毒16型E7癌基因与口腔鳞状细胞癌中Cdc25A的过表达相关。
Virchows Arch. 2007 Jan;450(1):65-71. doi: 10.1007/s00428-006-0327-x. Epub 2006 Nov 17.
4
Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts.对变异型人乳头瘤病毒16型E5蛋白诱导小鼠成纤维细胞有丝分裂能力的分析。
Cancer Cell Int. 2006 Aug 9;6:19. doi: 10.1186/1475-2867-6-19.
5
Cytotoxic-T-lymphocyte human papillomavirus type 16 E5 peptide with CpG-oligodeoxynucleotide can eliminate tumor growth in C57BL/6 mice.细胞毒性T淋巴细胞人乳头瘤病毒16型E5肽与CpG-寡脱氧核苷酸可消除C57BL/6小鼠体内的肿瘤生长。
J Virol. 2004 Feb;78(3):1333-43. doi: 10.1128/jvi.78.3.1333-1343.2004.
6
The human papillomavirus type 16 E5 protein impairs TRAIL- and FasL-mediated apoptosis in HaCaT cells by different mechanisms.人乳头瘤病毒16型E5蛋白通过不同机制损害TRAIL和FasL介导的HaCaT细胞凋亡。
J Virol. 2002 Dec;76(23):12162-72. doi: 10.1128/jvi.76.23.12162-12172.2002.

本文引用的文献

1
Analysis of human papillomavirus type 16 E5 oncogene expression in vitro and from bicistronic messenger RNAs.人乳头瘤病毒16型E5癌基因在体外及双顺反子信使核糖核酸中的表达分析
Intervirology. 1995;38(6):339-45. doi: 10.1159/000150461.
2
Detection of human papillomavirus type-16 DNA utilising microtitre-plate based amplification reactions and a solid-phase enzyme-immunoassay detection system.
J Virol Methods. 1996 Apr 26;58(1-2):59-69. doi: 10.1016/0166-0934(95)01988-x.
3
The E5 oncoprotein of human papillomavirus type 16 transforms fibroblasts and effects the downregulation of the epidermal growth factor receptor in keratinocytes.人乳头瘤病毒16型的E5癌蛋白可转化成纤维细胞，并影响角质形成细胞中表皮生长因子受体的下调。
J Virol. 1993 Aug;67(8):4521-32. doi: 10.1128/JVI.67.8.4521-4532.1993.
4
Use of semi-quantitative PCR for human papillomavirus DNA type 16 to identify women with high grade cervical disease in a population presenting with a mildly dyskaryotic smear report.运用半定量聚合酶链反应检测人乳头瘤病毒16型DNA，以在呈现轻度核异质涂片报告的人群中识别患有高级别宫颈疾病的女性。
Br J Cancer. 1993 Mar;67(3):602-5. doi: 10.1038/bjc.1993.110.
5
Rapid diagnosis of enterovirus infection by magnetic bead extraction and polymerase chain reaction detection of enterovirus RNA in clinical specimens.通过磁珠提取和聚合酶链反应检测临床标本中的肠道病毒RNA对肠道病毒感染进行快速诊断。
J Clin Microbiol. 1993 Jan;31(1):31-8. doi: 10.1128/jcm.31.1.31-38.1993.
6
Type-specific human papillomavirus DNA in abnormal smears as a predictor of high-grade cervical intraepithelial neoplasia.异常涂片检查中特定类型的人乳头瘤病毒DNA作为高级别宫颈上皮内瘤变的预测指标
Br J Cancer. 1994 Jan;69(1):167-71. doi: 10.1038/bjc.1994.28.
7
Human papillomavirus testing by hybrid capture appears to be useful in triaging women with a cytologic diagnosis of atypical squamous cells of undetermined significance.通过杂交捕获法进行人乳头瘤病毒检测，对于对意义不明确的非典型鳞状细胞进行细胞学诊断的女性分流似乎是有用的。
Am J Obstet Gynecol. 1995 Mar;172(3):946-54. doi: 10.1016/0002-9378(95)90026-8.
8
Integration of human papillomavirus type 16 DNA into the human genome leads to increased stability of E6 and E7 mRNAs: implications for cervical carcinogenesis.人乳头瘤病毒16型DNA整合入人类基因组导致E6和E7信使核糖核酸稳定性增加：对子宫颈癌发生的影响
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1654-8. doi: 10.1073/pnas.92.5.1654.
9
Transcription of papillomavirus genomes.乳头瘤病毒基因组的转录
Intervirology. 1994;37(3-4):159-67. doi: 10.1159/000150374.
10
Cellular proteins associated with the E5 oncoprotein of human papillomavirus type 16.与16型人乳头瘤病毒E5癌蛋白相关的细胞蛋白
Biochem Soc Trans. 1994 Aug;22(3):333S. doi: 10.1042/bst022333s.

通过逆转录聚合酶链反应检测人乳头瘤病毒16型早期基因转录与宫颈细胞学异常相关。

Detection of human papillomavirus type 16 early-gene transcription by reverse transcription-PCR is associated with abnormal cervical cytology.

作者信息

Biswas C, Kell B, Mant C, Jewers R J, Cason J, Muir P, Raju K S, Best J M

机构信息

Department of Gynaecology, UMDS St. Thomas' Campus, London, United Kingdom.

出版信息

J Clin Microbiol. 1997 Jun;35(6):1560-4. doi: 10.1128/jcm.35.6.1560-1564.1997.

DOI:10.1128/jcm.35.6.1560-1564.1997
PMID:9163482
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC229787/
Abstract

Human papillomavirus type 16 (HPV-16) is associated with abnormal Papanicolou smears, indicative of cervical intraepithelial neoplasia. HPV-16 is the most common genital HPV and is found in up to 40% of young women with normal cervical cytology. In order to investigate whether transcriptionally active HPV-16 infection is associated with abnormal cervical smears, a reverse transcription-nested PCR assay with primers from the E5 open reading frame was developed to detect all HPV-16 early-region mRNA (E-mRNA) transcripts. It was used to study HPV-16-infected women with normal and abnormal cervical cytologies to obtain evidence of active infection. Among HPV-16 DNA-positive women, HPV-16 E-mRNA was detected in 15 of 37 (40.5%) women with abnormal cervical cytology but in only 4 of 35 (11.4%) women with normal cytology (P = 0.007). Thus, HPV-16 E-mRNA transcription is associated with abnormal cervical smears and may have value as a prognostic marker of progressive disease.

摘要

16型人乳头瘤病毒（HPV-16）与巴氏涂片异常有关，提示宫颈上皮内瘤变。HPV-16是最常见的生殖道HPV，在宫颈细胞学正常的年轻女性中，高达40%可检测到该病毒。为了研究转录活跃的HPV-16感染是否与宫颈涂片异常有关，开发了一种使用E5开放阅读框引物的逆转录巢式PCR检测方法，以检测所有HPV-16早期区域mRNA（E-mRNA）转录本。该方法用于研究HPV-16感染且宫颈细胞学正常和异常的女性，以获取活跃感染的证据。在HPV-16 DNA阳性女性中，宫颈细胞学异常的37名女性中有15名（40.5%）检测到HPV-16 E-mRNA，而宫颈细胞学正常的35名女性中只有4名（11.4%）检测到（P = 0.007）。因此，HPV-16 E-mRNA转录与宫颈涂片异常有关，可能作为疾病进展的预后标志物具有价值。