Pärsson H, Nässberger L, Thörne J, Norgren L
Department of Surgery, Lund University, Sweden.
J Biomed Mater Res. 1995 Apr;29(4):519-25. doi: 10.1002/jbm.820290412.
The metabolism of granulocytes as well as platelets evoked by incubation with different synthetic vascular grafts was monitored during 6-h batch experiments using microcalorimetry. Standard knitted Dacron grafts, ePTFE-grafts, knitted Dacron grafts with collagen impregnation, and knitted Dacron grafts with external collagen-coating were used. The heat production per cell was calculated. A rapid increase of metabolic activity followed by a gradual decrease was demonstrated with both granulocyte suspension and platelet concentrate. Significant differences were obtained between the materials with a maximum response of Dacron grafts with collagen impregnation for both granulocyte and platelet response. The materials had different surface morphologies regarding cell adhesion after incubation as demonstrated with scanning electron microscopy with more pronounced adhesion on the collagen-impregnated grafts. The results suggest that microcalorimetry may be useful for the evaluation of cellular reactions on different biomaterials. However, further studies have to reveal the specificity of the reactions.
在6小时的批量实验中，使用微量热法监测了与不同合成血管移植物孵育后粒细胞以及血小板的代谢情况。使用了标准针织涤纶移植物、ePTFE移植物、浸有胶原蛋白的针织涤纶移植物以及外部涂有胶原蛋白的针织涤纶移植物。计算了每个细胞的产热。粒细胞悬液和血小板浓缩液均显示出代谢活性迅速增加，随后逐渐下降。不同材料之间存在显著差异，浸有胶原蛋白的涤纶移植物对粒细胞和血小板反应的最大反应最为明显。如扫描电子显微镜所示，孵育后材料在细胞黏附方面具有不同的表面形态，浸有胶原蛋白的移植物上的黏附更为明显。结果表明，微量热法可能有助于评估不同生物材料上的细胞反应。然而，进一步的研究必须揭示这些反应的特异性。
