表皮生长因子（EGF）和氯化钾（KCl）去极化对PC12细胞神经突生长的刺激作用：一种不依赖钙离子（Ca(2+)）的现象
Stimulation of neurite outgrowth in PC12 cells by EGF and KCl depolarization: a Ca(2+)-independent phenomenon.
作者信息
Mark M D, Liu Y, Wong S T, Hinds T R, Storm D R
机构信息
Department of Pharmacology, University of Washington, Seattle 98195, USA.
出版信息
J Cell Biol. 1995 Aug;130(3):701-10. doi: 10.1083/jcb.130.3.701.
Abstract
MAP kinase activity is necessary for growth factor induction of neurite outgrowth in PC12 cells. Although NGF and EGF both stimulate MAP kinase activity, EGF does not stimulate neurite extension. We report that EGF, in combination with KCl, stimulates neurite outgrowth in PC12 cells. This phenomenon was independent of intracellular Ca2+ increases and not due to enhancement of MAP kinase activity over that seen with EGF alone. However, EGF plus KCl increased intracellular cAMP, and other cAMP elevating agents acted synergistically with EGF to promote neurite outgrowth. Stimulation of neurite outgrowth by cAMP and EGF was blocked by inhibitors of transcription suggesting that synergistic regulation of transcription by the cAMP and MAP kinase pathways may stimulate neurite growth.
摘要
丝裂原活化蛋白激酶（MAP激酶）活性对于PC12细胞中生长因子诱导的神经突生长是必需的。尽管神经生长因子（NGF）和表皮生长因子（EGF）都能刺激MAP激酶活性，但EGF并不刺激神经突延伸。我们报告，EGF与氯化钾（KCl）联合使用时，可刺激PC12细胞的神经突生长。这种现象与细胞内钙离子（Ca2+）增加无关，也不是由于MAP激酶活性比单独使用EGF时增强所致。然而，EGF加KCl可增加细胞内环磷酸腺苷（cAMP），其他能提高cAMP水平的试剂与EGF协同作用以促进神经突生长。cAMP和EGF对神经突生长的刺激被转录抑制剂阻断，这表明cAMP和MAP激酶途径对转录的协同调节可能刺激神经突生长。
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本文引用的文献
1
Epidermal growth factor and transforming growth factor-alpha can induce neuronal differentiation of rat pheochromocytoma PC12 cells under particular culture conditions.
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2
Transcription factor p91 interacts with the epidermal growth factor receptor and mediates activation of the c-fos gene promoter.转录因子p91与表皮生长因子受体相互作用，并介导c-fos基因启动子的激活。
Cell. 1993 Sep 24;74(6):1135-45. doi: 10.1016/0092-8674(93)90734-8.
3
The cytoplasmic raf oncogene induces a neuronal phenotype in PC12 cells: a potential role for cellular raf kinases in neuronal growth factor signal transduction.细胞质raf癌基因在PC12细胞中诱导神经元表型：细胞raf激酶在神经营养因子信号转导中的潜在作用。
Proc Natl Acad Sci U S A. 1993 Jun 1;90(11):5016-20. doi: 10.1073/pnas.90.11.5016.
4
Pertussis toxin can distinguish the augmentary effect elicited by epidermal growth factor from that of phorbol ester on luteal adenylate cyclase activity.
Endocrinology. 1993 Jul;133(1):265-70. doi: 10.1210/endo.133.1.8319575.
5
Nerve growth factor activates a Ras-dependent protein kinase that stimulates c-fos transcription via phosphorylation of CREB.神经生长因子激活一种依赖Ras的蛋白激酶，该激酶通过磷酸化CREB来刺激c-fos转录。
Cell. 1994 Jun 3;77(5):713-25. doi: 10.1016/0092-8674(94)90055-8.
6
Regulation of CREB phosphorylation in the suprachiasmatic nucleus by light and a circadian clock.光和生物钟对视交叉上核中CREB磷酸化的调节。
Science. 1993 Apr 9;260(5105):238-41. doi: 10.1126/science.8097062.
7
Membrane depolarization and calcium influx stimulate MEK and MAP kinase via activation of Ras.膜去极化和钙内流通过激活Ras刺激MEK和丝裂原活化蛋白激酶。
Neuron. 1994 Jun;12(6):1207-21. doi: 10.1016/0896-6273(94)90438-3.
8
Type I adenylyl cyclase functions as a coincidence detector for control of cyclic AMP response element-mediated transcription: synergistic regulation of transcription by Ca2+ and isoproterenol.I型腺苷酸环化酶作为一种用于控制环磷酸腺苷反应元件介导转录的巧合探测器：Ca2+和异丙肾上腺素对转录的协同调节。
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9
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10
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J Biol Chem. 1994 Oct 14;269(41):25699-709.
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