Ginty D D, Bonni A, Greenberg M E
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Cell. 1994 Jun 3;77(5):713-25. doi: 10.1016/0092-8674(94)90055-8.
A mechanism by which the nerve growth factor (NGF) signal is transduced to the nucleus to induce gene expression has been characterized. An NGF-inducible, Ras-dependent protein kinase has been identified that catalyzes the phosphorylation of the cyclic AMP response element-binding protein (CREB) at Ser-133. Phosphorylation of Ser-133 stimulates the ability of CREB to activate transcription in NGF-treated cells. These findings suggest that CREB has a more widespread function than previously believed and functions in the nucleus as a general mediator of growth factor responses.
一种将神经生长因子（NGF）信号转导至细胞核以诱导基因表达的机制已得到阐明。已鉴定出一种NGF诱导的、Ras依赖性蛋白激酶，它催化环磷酸腺苷反应元件结合蛋白（CREB）在Ser-133处的磷酸化。Ser-133的磷酸化增强了CREB在NGF处理细胞中激活转录的能力。这些发现表明，CREB具有比先前认为的更广泛的功能，并且在细胞核中作为生长因子反应的一般介导因子发挥作用。
