Rowen J L, Smith C W, Edwards M S
Section of Infectious Diseases, Baylor College of Medicine, Houston, Texas 77030, USA.
J Infect Dis. 1995 Aug;172(2):420-6. doi: 10.1093/infdis/172.2.420.
Neonatal monocytes have diminished function compared with adult cells. The ability to recruit neutrophils through elaboration of chemoattractants has not been evaluated in humans. The pattern of chemoattractant release induced by group B streptococci (GBS) also is unknown. Adult and cord blood monocytes were stimulated with GBS. Supernatants were used as the attractant in blind well chambers; migration to neonatal supernatants was diminished. Interleukin-8 (IL-8) and leukotriene B4 (LTB4) were released in greater quantities by adult monocytes in response to either GBS or lipopolysaccharide. Opsonization of GBS was not required for IL-8 release. Adult monocytes released more LTB4 when stimulated with unopsonized GBS than with opsonized GBS; the neonate's LTB4 production did not increase. IL-8 and LTB4 accounted for the majority of chemoattractant activity released in response to GBS. Decreased production of LTB4 and IL-8 may contribute to the neonate's poor host response to GBS.
与成人细胞相比，新生儿单核细胞功能有所减退。在人类中，通过分泌趋化因子招募中性粒细胞的能力尚未得到评估。B族链球菌（GBS）诱导的趋化因子释放模式也不清楚。用GBS刺激成人和脐血单核细胞。将上清液用作盲孔小室中的趋化剂；向新生儿上清液的迁移减少。成人单核细胞对GBS或脂多糖反应时，会释放更多的白细胞介素-8（IL-8）和白三烯B4（LTB4）。IL-8释放不需要GBS的调理作用。未调理的GBS刺激时，成人单核细胞释放的LTB4比调理后的GBS更多；新生儿的LTB4产量没有增加。IL-8和LTB4占GBS刺激释放的趋化活性的大部分。LTB4和IL-8产量降低可能导致新生儿对GBS的宿主反应较差。
