Terai M, Miwa K, Williams T, Kabat W, Fukuyama M, Okajima Y, Igarashi H, Shulman S T
Division of Infectious Diseases, Children's Memorial Hospital, Chicago, IL 60614, USA.
J Infect Dis. 1995 Aug;172(2):558-61. doi: 10.1093/infdis/172.2.558.
To detect a causative superantigen and to clarify a possible role for staphylococci in Kawasaki disease (KD), culture supernatants of individual bacterial isolates from 11 acute-stage patients were studied. Toxic shock syndrome toxin-1 (TSST-1) and antibody to TSST-1 and enterotoxins A (SEA), B (SEB), and C (SEC) in acute (mean, day 7) and late convalescent (mean, month 15) sera from 26 patients (12 with coronary artery aneurysms) and 22 age-matched controls were measured. Only 1 of 60 supernatants was mitogenic for human lymphocytes; it was 1 of the 4 Staphylococcus aureus isolates. Mitogenicity was neutralized by sera obtained after administration of intravenous gamma globulin (mean, week 4) but not by late convalescent sera. TSST-1 was detectable in 2 of 26 acute sera and 1 of 22 control sera. No KD but 1 control serum had IgM to TSST-1. IgG seroconversion rates to TSST-1, SEA, SEB, and SEC were 10%, 15%, 21% and 16%, respectively. These data do not support the involvement of toxin-producing staphylococci in KD.
为检测致病超抗原并阐明葡萄球菌在川崎病（KD）中可能的作用，对11例急性期患者的单个细菌分离株的培养上清液进行了研究。检测了26例患者（12例有冠状动脉瘤）和22例年龄匹配对照的急性（平均第7天）和恢复期晚期（平均第15个月）血清中的中毒性休克综合征毒素-1（TSST-1）以及TSST-1和肠毒素A（SEA）、B（SEB）和C（SEC）的抗体。60份上清液中只有1份对人淋巴细胞有促有丝分裂作用；它是4株金黄色葡萄球菌分离株中的1株。促有丝分裂作用被静脉注射丙种球蛋白（平均第4周）后获得的血清中和，但未被恢复期晚期血清中和。26份急性血清中有2份可检测到TSST-1，22份对照血清中有1份可检测到。KD患者血清中无TSST-1 IgM，但1份对照血清中有。TSST-1、SEA、SEB和SEC的IgG血清转化率分别为10%、15%、21%和16%。这些数据不支持产毒素葡萄球菌参与KD的发病。
