Okuda Y, Sawada T, Mizutani M, Sone H, Suzuki S, Kawakami Y, Soma M, Suzuki M, Yaoita H, Yamashita K
Department of Internal Medicine, University of Tsukuba, Japan.
Life Sci. 1995;57(5):PL71-4. doi: 10.1016/0024-3205(95)00287-g.
The effects of elevated glucose and eicosapentaenoic acid (EPA, C20:5 omega 3) on myo-inositol uptake in human skin fibroblasts (HSF) were evaluated. Myo-inositol incorporation into HSF was dependent on an active transport system via Na(+)-K+ ATPase activity based on the results with Na+ deprivation and ouabain (5 mM). Although glucose (27.5, 55 mM) inhibited 2-[3H] myo-inositol uptake, the addition of EPA (3 x 10(-4) M) prevented glucose-mediated inhibition. Since EPA decreased glucose-mediated inhibition of myo-inositol uptake, this agent might ameliorate some of the devastating functions associated with diabetes.
评估了高糖和二十碳五烯酸（EPA，C20:5 ω-3）对人皮肤成纤维细胞（HSF）肌醇摄取的影响。基于用钠剥夺和哇巴因（5 mM）得到的结果，肌醇掺入HSF依赖于经由钠钾ATP酶活性的主动转运系统。尽管葡萄糖（27.5、55 mM）抑制了2-[3H]肌醇摄取，但添加EPA（3×10⁻⁴ M）可防止葡萄糖介导的抑制作用。由于EPA降低了葡萄糖介导的肌醇摄取抑制作用，该药物可能会改善一些与糖尿病相关的破坏性功能。
