Zenklusen J C, Weitzel J N, Ball H G, Conti C J
Department of Carcinogenesis, University of Texas M.D. Anderson Cancer Center, Smithville 78957, USA.
Oncogene. 1995 Jul 20;11(2):359-63.
We studied loss of heterozygosity (LOH) in chromosome 7q in order to determine the location of a putative tumor suppressor gene (TSG) in human epithelial ovarian carcinomas. Samples were obtained from 26 primary ovarian carcinomas at the time of staging laparotomy. Paired normal and tumoral DNAs were used as templates for polymerase chain reaction amplification of a set of 14 (C-A)n microsatellite repeats on 7q21-qter. All the cases studied presented LOH at one or more loci on 7q. Seventy-three percent LOH (in 14 of 19 informative cases) were detected in D7S522 at 7q31.1. The percentages of LOH were normally distributed around microsatellite D7S522 determining a smallest common deleted region of 1 cM. The high incidence of LOH in primary ovarian carcinomas suggests that a TSG relevant to the development of ovarian cancers is present at 7q31.1, confirming our previous functional evidence for a TSG in this region.
我们研究了7号染色体长臂（7q）上的杂合性缺失（LOH），以确定人类上皮性卵巢癌中一个假定的肿瘤抑制基因（TSG）的位置。样本取自26例原发性卵巢癌患者分期剖腹手术时。配对的正常和肿瘤DNA用作模板，对7q21 - qter上一组14个（C - A）n微卫星重复序列进行聚合酶链反应扩增。所有研究病例在7q的一个或多个位点出现了LOH。在7q31.1的D7S522位点检测到73%的LOH（19例信息充分的病例中有14例）。LOH的百分比围绕微卫星D7S522呈正态分布，确定了一个最小的共同缺失区域为1厘摩。原发性卵巢癌中LOH的高发生率表明，与卵巢癌发生相关的一个TSG存在于7q31.1，证实了我们之前关于该区域存在TSG的功能证据。
