Zenklusen J C, Thompson J C, Troncoso P, Kagan J, Conti C J
Department of Carcinogenesis, University of Texas M. D. Anderson Cancer Center, Smithville 78957.
Cancer Res. 1994 Dec 15;54(24):6370-3.
We studied loss of heterozygosity (LOH) on human chromosome 7q to determine the location of a putative tumor suppressor gene (TSG) in human primary prostate carcinomas. Samples were obtained from 16 primary prostate carcinomas surgically removed from patients at The University of Texas M. D. Anderson Cancer Center. Paired normal and tumor DNAs were used as template for PCR amplification of a set of 14 CA microsatellite repeats on 7q21-qter. Twelve of 16 cases studied had LOH at one or more loci on 7q. Eighty-three percent LOH (five of six informative cases) was detected with D7S522 at 7q31.1-7q31.2. Percentage of LOH was normally distributed around D7S522. The high incidence of LOH in primary prostate carcinomas suggests that there is a TSG relevant to the development of prostate cancers at 7q31.1-31.2, confirming our previous functional evidence for a TSG at this location. Further research needs to be conducted to establish the identity and function of this putative TSG.
我们研究了人类7号染色体q臂上的杂合性缺失（LOH），以确定人类原发性前列腺癌中一个假定的肿瘤抑制基因（TSG）的位置。样本取自德克萨斯大学MD安德森癌症中心接受手术切除的16例原发性前列腺癌患者。以配对的正常DNA和肿瘤DNA作为模板，对7q21 - qter上的一组14个CA微卫星重复序列进行PCR扩增。在研究的16例病例中，有12例在7q的一个或多个位点存在LOH。在7q31.1 - 7q31.2处的D7S522检测到83%的LOH（6例信息充分的病例中有5例）。LOH的百分比围绕D7S522呈正态分布。原发性前列腺癌中LOH的高发生率表明，在7q31.1 - 31.2处存在一个与前列腺癌发生相关的TSG，这证实了我们之前关于该位置存在TSG的功能证据。需要进一步研究以确定这个假定的TSG的身份和功能。
