Bredt D S, Wang T L, Cohen N A, Guggino W B, Snyder S H
Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6753-7. doi: 10.1073/pnas.92.15.6753.
We have cloned two inwardly rectifying K+ channels that occur selectively in neurons in the brain and are designated BIRK (brain inwardly rectifying K+) channels. BIRK1 mRNA is extremely abundant and is enriched in specific brainstem nuclei, BIRK1 displays a consensus phosphate-binding loop, and expression in Xenopus oocytes has shown that its conductance is inhibited by ATP and adenosine 5'-[gamma-thio]triphosphate. BIRK2 is far less abundant and is selectively localized in telencephalic neurons. BIRK2 has a consensus sequence for cAMP-dependent phosphorylation.
我们克隆了两种内向整流钾通道，它们选择性地出现在大脑神经元中，被命名为BIRK（脑内向整流钾）通道。BIRK1 mRNA极为丰富，且在特定脑干核中富集，BIRK1显示出一个共有磷酸结合环，在非洲爪蟾卵母细胞中的表达表明其电导受到ATP和腺苷5'-[γ-硫代]三磷酸的抑制。BIRK2的丰度要低得多，且选择性地定位于端脑神经元中。BIRK2具有cAMP依赖性磷酸化的共有序列。
