Sablowski R W, Baulcombe D C, Bevan M
Molecular Genetics Department, John Innes Centre, Norwich, United Kingdom.
Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):6901-5. doi: 10.1073/pnas.92.15.6901.
The promoter of the bean PAL2 gene (encoding phenylalanine ammonia-lyase; EC 4.3.1.5) is a model for studies of tissue-restricted gene expression in plants. Petal epidermis is one of the tissues in which this promoter is activated in tobacco. Previous work suggested that a major factor establishing the pattern of PAL2 expression in tobacco petals is the tissue distribution of a protein closely related to Myb305, which is a Myb-like transcriptional activator from snapdragon. In the present work, we show that Myb305 expression in tobacco leaves causes ectopic activation of the PAL2 promoter. To achieve Myb305 expression in planta, a viral expression vector was used. This approach combines the utility of transient assays with the possibility of direct biochemical detection of the introduced factor and may have wider application for studying the function of plant transcription factors.
豆类PAL2基因（编码苯丙氨酸解氨酶；EC 4.3.1.5）的启动子是研究植物组织特异性基因表达的一个模型。花瓣表皮是该启动子在烟草中被激活的组织之一。先前的研究表明，在烟草花瓣中建立PAL2表达模式的一个主要因素是一种与Myb305密切相关的蛋白质的组织分布，Myb305是一种来自金鱼草的Myb样转录激活因子。在本研究中，我们发现烟草叶片中Myb305的表达会导致PAL2启动子的异位激活。为了在植物中实现Myb305的表达，使用了一种病毒表达载体。这种方法将瞬时分析的实用性与对导入因子进行直接生化检测的可能性结合起来，可能在研究植物转录因子的功能方面有更广泛的应用。
