Mele Giovanni, Ori Naomi, Sato Yutaka, Hake Sarah
Plant Gene Expression Center, United States Department of Agriculture-Agricultural Research Service, Albany, CA 94710, USA.
Genes Dev. 2003 Sep 1;17(17):2088-93. doi: 10.1101/gad.1120003. Epub 2003 Aug 15.
Members of the KNOX gene family have important roles in plant meristems by regulating cell division and differentiation. BREVIPEDICELLUS (BP), one of seven KNOX genes in Arabidopsis, has a primary role in internode patterning. We carried out a comparison of RNA expression profiles between wild-type seedlings and bp mutants at a developmental stage prior to a visible phenotypic difference. Transcript differences were found for a number of genes in cell wall biosynthesis, especially genes in the lignin pathway. The regulation of lignin biosynthesis by BP was demonstrated by observing increased lignin deposition in bp mutants following bolting, decreased lignification in plants overexpressing BP, and aberrant lignin deposition in discrete regions of the bp stem. Furthermore, we showed that BP binds promoters of some genes in the lignin pathway. Our results provide a metabolic fingerprint for BP and identify the lignin pathway as one of the coordinate processes that BP regulates.
KNOX基因家族成员通过调节细胞分裂和分化在植物分生组织中发挥重要作用。拟南芥七个KNOX基因之一的短柄(BREVIPEDICELLUS,BP)在节间模式形成中起主要作用。我们在野生型幼苗和bp突变体之间在可见表型差异之前的发育阶段进行了RNA表达谱比较。发现细胞壁生物合成中的许多基因存在转录差异,尤其是木质素途径中的基因。通过观察bp突变体抽薹后木质素沉积增加、过表达BP的植物中木质化减少以及bp茎的离散区域中异常的木质素沉积,证明了BP对木质素生物合成的调节作用。此外,我们表明BP结合木质素途径中一些基因的启动子。我们的结果为BP提供了一个代谢指纹,并将木质素途径确定为BP调节的协同过程之一。