Campfield L A, Smith F J, Guisez Y, Devos R, Burn P
Department of Metabolic Diseases, Hoffmann-La Roche Incorporated, Nutley, NJ 07110, USA.
Science. 1995 Jul 28;269(5223):546-9. doi: 10.1126/science.7624778.
The recent positional cloning of the mouse ob gene and its human homology has provided the basis to investigate the potential role of the ob gene product in body weight regulation. A biologically active form of recombinant mouse OB protein was overexpressed and purified to near homogeneity from a bacterial expression system. Peripheral and central administration of microgram doses of OB protein reduced food intake and body weight of ob/ob and diet-induced obese mice but not in db/db obese mice. The behavioral effects after brain administration suggest that OB protein can act directly on neuronal networks that control feeding and energy balance.
最近对小鼠ob基因及其人类同源基因的定位克隆为研究ob基因产物在体重调节中的潜在作用提供了基础。一种具有生物活性的重组小鼠OB蛋白在细菌表达系统中过表达并纯化至接近均一性。外周和中枢给予微克剂量的OB蛋白可降低ob/ob小鼠和饮食诱导肥胖小鼠的食物摄入量和体重，但对db/db肥胖小鼠无效。脑内给药后的行为学效应表明，OB蛋白可直接作用于控制进食和能量平衡的神经网络。
