Pelleymounter M A, Cullen M J, Baker M B, Hecht R, Winters D, Boone T, Collins F
Department of Neurobiology, Amgen, Inc., Thousand Oaks, CA 91320, USA.
Science. 1995 Jul 28;269(5223):540-3. doi: 10.1126/science.7624776.
C57BL/6J mice with a mutation in the obese (ob) gene are obese, diabetic, and exhibit reduced activity, metabolism, and body temperature. Daily intraperitoneal injection of these mice with recombinant OB protein lowered their body weight, percent body fat, food intake, and serum concentrations of glucose and insulin. In addition, metabolic rate, body temperature, and activity levels were increased by this treatment. None of these parameters was altered beyond the level observed in lean controls, suggesting that the OB protein normalized the metabolic status of the ob/ob mice. Lean animals injected with OB protein maintained a smaller weight loss throughout the 28-day study and showed no changes in any of the metabolic parameters. These data suggest that the OB protein regulates body weight and fat deposition through effects on metabolism and appetite.
肥胖（ob）基因发生突变的C57BL/6J小鼠肥胖、患糖尿病，且活动量、代谢率和体温降低。每天给这些小鼠腹腔注射重组OB蛋白可降低它们的体重、体脂百分比、食物摄入量以及血糖和胰岛素的血清浓度。此外，这种治疗可提高代谢率、体温和活动水平。这些参数均未改变至超过瘦素对照组所观察到的水平，这表明OB蛋白使ob/ob小鼠的代谢状态恢复正常。在整个28天的研究中，注射OB蛋白的瘦小鼠体重减轻幅度较小，且任何代谢参数均无变化。这些数据表明，OB蛋白通过影响代谢和食欲来调节体重和脂肪沉积。
