1988 - 1992年布鲁克林淋病奈瑟菌分离株对青霉素和四环素的敏感性

Susceptibility of isolates of Neisseria gonorrhoeae to penicillin and tetracycline in Brooklyn, 1988-1992.

作者信息

Cummings M C, Covino J M, Smith B L, Ratiu E S, Draft K, McCormack W M

机构信息

Department of Medicine, State University of New York Health Science Center at Brooklyn 11203, USA.

出版信息

Sex Transm Dis. 1995 Mar-Apr;22(2):110-3. doi: 10.1097/00007435-199503000-00006.

DOI:10.1097/00007435-199503000-00006

PMID:7624811

Abstract

BACKGROUND AND OBJECTIVES

The aim of this analysis was to determine the rate of resistance of Neisseria gonorrhoeae to penicillin and tetracycline over 5 years.

STUDY DESIGN

The authors studied 500 isolates of N. gonorrhoeae. Minimum inhibitory concentrations of various antimicrobial agents, including penicillins, tetracyclines, quinolones, cephalosporins, spectinomycin, and trospectomycin, were determined using agar dilution. Organisms that produced beta-lactamase were classified as penicillinase-producing N. gonorrhoeae, and those with tetracycline minimum inhibitory concentrations > 16 micrograms/ml were considered presumptive high-level tetracycline resistant. Organisms with Minimum inhibitory concentrations > 2.0 micrograms/ml were presumptively considered to have chromosomally mediated resistance to penicillin or tetracycline. Isolates with none of these forms of resistance were considered susceptible to penicillin and tetracycline.

RESULTS

Penicillinase-producing N. gonorrhoeae represented 34.7%, 40.7%, and 44.9% of gonococcal isolates in 1988, 1989, and 1990, respectively. Only 14.3% and 15.0% of the isolates in 1991 and 1992 were penicillinase-producing N. gonorrhoeae. In 1988, 1.0% of isolates were chromosomally mediated penicillin resistant. In contrast, chromosomally mediated penicillin resistant gonococci represented 7.5% to 22.4% of isolates from 1989 to 1992. In 1988, 26.0% of isolates were high-level tetracycline resistant. The prevalence of presumptive high-level tetracycline resistant organisms decreased after 1988. From 1989 to 1992, only 8.2% to 14.8% of gonococcal isolates were presumptive high-level tetracycline resistant. No chromosomally mediated tetracycline resistant isolates were identified in 1988. In 1989 and 1990, 11.6% and 10.2%, respectively, of isolates were chromosomally mediated tetracycline resistant. Chromosomally mediated tetracycline resistant N. gonorrhoeae represented 2.0% of isolates in 1991 and 25.0% of isolates in 1992. All isolates tested were susceptible to the other antibiotics.

CONCLUSION

Continued surveillance of sensitivity of contemporary gonococci to antimicrobial agents is important.

摘要

背景与目的

本分析旨在确定5年间淋病奈瑟菌对青霉素和四环素的耐药率。

研究设计

作者研究了500株淋病奈瑟菌。使用琼脂稀释法测定了包括青霉素、四环素、喹诺酮类、头孢菌素类、壮观霉素和曲古霉素在内的多种抗菌药物的最低抑菌浓度。产生β-内酰胺酶的菌株被分类为产青霉素酶淋病奈瑟菌，四环素最低抑菌浓度>16微克/毫升的菌株被认为是推定的高水平四环素耐药菌。最低抑菌浓度>2.0微克/毫升的菌株被推定为对青霉素或四环素具有染色体介导的耐药性。没有这些耐药形式的菌株被认为对青霉素和四环素敏感。

结果

产青霉素酶淋病奈瑟菌分别占1988年、1989年和1990年淋球菌分离株的34.7%、40.7%和44.9%。1991年和1992年分别只有14.3%和15.0%的分离株是产青霉素酶淋病奈瑟菌。1988年，1.0%的分离株具有染色体介导的青霉素耐药性。相比之下，1989年至1992年，染色体介导的青霉素耐药淋球菌占分离株的7.5%至22.4%。1988年，26.0%的分离株是高水平四环素耐药菌。1988年后，推定的高水平四环素耐药菌的患病率下降。1989年至1992年，只有8.2%至14.8%的淋球菌分离株是推定的高水平四环素耐药菌。1988年未鉴定出染色体介导的四环素耐药分离株。1989年和1990年，分别有11.6%和10.2%的分离株是染色体介导的四环素耐药菌。染色体介导的四环素耐药淋病奈瑟菌在1991年占分离株的2.0%，在1992年占分离株的25.0%。所有测试的分离株对其他抗生素敏感。