Granelli-Piperno A, Vassalli J D, Reich E
J Exp Med. 1979 Jan 1;149(1):284-9. doi: 10.1084/jem.149.1.284.
Polymorphonuclear leukocytes purified from human peripheral blood synthesized RNA and proteins when placed in cell culture. Autoradiography of the cultured cells revealed that a majority of mature PMNs were engaged in macromolecule synthesis, and an analysis of newly synthesized proteins by SDS-polyacrylamide gel electrophoresis showed that many different polypeptide chains were synthesized by these cells. The rate of [3H]uridine incorporation and the pattern of newly synthesized proteins were modulated by Con A and glucocorticoids. These results suggest that in spite of their short lifetime and a large performed enzymatic apparatus, mature PMNs retain a substantial capacity for RNA and protein synthesis; and, further, that modulation of macromolecule synthesis forms part of the mechanism by which PMNs respond to inflammatory and anti-flammatory stimuli.
从人外周血中纯化的多形核白细胞置于细胞培养中时可合成RNA和蛋白质。对培养细胞进行放射自显影显示，大多数成熟的多形核白细胞参与大分子合成，通过SDS-聚丙烯酰胺凝胶电泳对新合成蛋白质的分析表明，这些细胞可合成许多不同的多肽链。伴刀豆球蛋白A和糖皮质激素可调节[3H]尿苷掺入率和新合成蛋白质的模式。这些结果表明，尽管成熟的多形核白细胞寿命较短且具有大量现成的酶装置，但它们仍保留了相当大的RNA和蛋白质合成能力；此外，大分子合成的调节是多形核白细胞对炎症和抗炎刺激作出反应的机制的一部分。
