Humphreys J M, Hughes V, Edwards S W
Department of Biochemistry, University of Liverpool, U.K.
Biochem Pharmacol. 1989 Apr 15;38(8):1241-6. doi: 10.1016/0006-2952(89)90329-8.
Treatment of human, peripheral blood neutrophils with gamma-interferon both "primed" their ability to generate reactive oxidants and increased their rate of protein synthesis. This increased rate of protein synthesis was greatest 60 min after the addition of 100 U/ml gamma-interferon and was not due to an increased intracellular pool of radiolabelled amino acid. Analysis of the newly-synthesized polypeptides by two-dimensional polyacrylamide gel electrophoresis (PAGE) revealed two classes of proteins which were regulated by this agent. The first of these represented proteins whose rate of labelling increased very little (1-2-fold) whereas the rate of biosynthesis of a second group of proteins increased more markedly (10-20-fold). We propose that these newly-synthesized, gamma-interferon regulated proteins play an important role in the function of these cells during an acute inflammatory response.
用γ-干扰素处理人外周血中性粒细胞，既能“启动”其产生活性氧化剂的能力，又能提高其蛋白质合成速率。在加入100 U/mlγ-干扰素后60分钟，这种蛋白质合成速率的增加最为显著，且这并非由于细胞内放射性标记氨基酸池的增加所致。通过二维聚丙烯酰胺凝胶电泳（PAGE）对新合成的多肽进行分析，发现有两类蛋白质受该因子调控。其中第一类蛋白质的标记速率增加很少（1 - 2倍），而第二类蛋白质的生物合成速率增加更为显著（10 - 20倍）。我们认为，这些新合成的、受γ-干扰素调控的蛋白质在急性炎症反应期间这些细胞的功能中发挥重要作用。
