McBride W H, Bard J B
J Exp Med. 1979 Feb 1;149(2):507-15. doi: 10.1084/jem.149.2.507.
A variety of adherent sarcoma, carcinoma and normal cells are surrounded in vitro by thick, transparent zones (approximately equal to 9 micron thick) that spleen cells and a variety of other cells and particles cannot penetrate. Seven lymphoblastoid cell lines did not possess such halos. The presence of these halos around adherent fibrosarcoma cells appeared to protect them from lymphocyte-mediated cytolysis. Hyaluronidase treatment, which destroyed the halo and allowed lymphocytes to approach the tumor cell membrane, enhanced the cytotoxic action of immune but not of normal spleen cells. These observations, in addition to highlighting a little-known feature of the cell surface, may also be of general relevance to the in vitro and in vivo killing of tumor cells by immune effector cells.
多种贴壁肉瘤细胞、癌细胞和正常细胞在体外被厚厚的透明带（约9微米厚）包围，脾细胞以及多种其他细胞和颗粒无法穿透该透明带。七个淋巴母细胞样细胞系不具有这种晕环。贴壁纤维肉瘤细胞周围存在这些晕环似乎可保护它们免受淋巴细胞介导的细胞溶解。透明质酸酶处理破坏了晕环，使淋巴细胞能够接近肿瘤细胞膜，增强了免疫脾细胞而非正常脾细胞的细胞毒性作用。这些观察结果除了突出细胞表面一个鲜为人知的特征外，可能还与免疫效应细胞在体外和体内杀伤肿瘤细胞具有普遍相关性。
