Banks J A, Hemming N, Poole J
International Blood Group Reference Laboratory, Bristol, UK.
Vox Sang. 1995;68(3):177-82. doi: 10.1111/j.1423-0410.1995.tb03923.x.
Ten red cell samples lacking the high incidence Gya antigen were found to have the previously undescribed Do(a-b-) phenotype. Fifteen Hy- red cell samples were Do(a-b+) with weak expression of Dob and 6 Jo(a-) red cell samples were Do(a+) with weak expression of Doa. Five of the 6 Jo(a-) samples had extremely weak expression of Dob. The sixth Jo(a-) was Do(b-). Immune precipitates were prepared from radio-iodinated antigen-positive red cells with anti-Gya, -Hy, -Doa and Dob. Immunoblotting of these immune precipitates with affinity-purified anti-Gya and anti-Dob under non-reducing conditions revealed similar broadly migrating bands of M(r) 48,700-59,750, suggesting that the Doa and Dob antigens are carried on the same glycoprotein as Gya and Hy. The phenotypically associated high incidence Joa antigen has previously been shown to reside on the Gya/Hy glycoprotein. Enzyme-treated and chemically modified red cells tested with anti-Doa, -Dob, -Gya, -Hy and -Joa gave the same pattern of reactivity. We propose that Gya, Hy and Joa become part of the Dombrock blood group system and that, henceforth, the Gya/Hy-active glycoprotein be renamed the Dombrock-active glycoprotein. The Gy(a)-Hy- Jo(a-) phenotype constitutes the 'null' phenotype within the Dombrock system.
发现10份缺乏高频率Gya抗原的红细胞样本具有之前未描述过的Do(a-b-)表型。15份Hy-红细胞样本为Do(a-b+)，Dob表达较弱；6份Jo(a-)红细胞样本为Do(a+)，Doa表达较弱。6份Jo(a-)样本中有5份Dob表达极弱。第6份Jo(a-)样本为Do(b-)。用抗Gya、-Hy、-Doa和-Dob从放射性碘化抗原阳性红细胞制备免疫沉淀物。在非还原条件下，用亲和纯化的抗Gya和抗Dob对这些免疫沉淀物进行免疫印迹，显示出类似的迁移较宽的条带，其相对分子质量为48,700 - 59,750，表明Doa和Dob抗原与Gya和Hy存在于同一糖蛋白上。之前已证明表型相关的高频率Joa抗原存在于Gya/Hy糖蛋白上。用抗Doa、-Dob、-Gya、-Hy和-Joa对酶处理和化学修饰的红细胞进行检测，得到相同的反应模式。我们提出Gya、Hy和Joa应成为达蒙布罗克血型系统的一部分，因此，Gya/Hy活性糖蛋白应重新命名为达蒙布罗克活性糖蛋白。Gy(a)-Hy- Jo(a-)表型构成达蒙布罗克系统中的“null”表型。
