Spring F A, Reid M E, Nicholson G
South Western Regional Transfusion Centre, Bristol, UK.
Vox Sang. 1994;66(1):72-7. doi: 10.1111/j.1423-0410.1994.tb00281.x.
The phenotypic association between the non-assigned high-incidence antigen Joa and the Gya collection antigens Gya and Hy was investigated by haemagglutination studies, flow cytometric analysis, immune precipitation and immunoblotting experiments. In haemagglutination tests anti-Joa gave the same pattern of reactivity with erythrocytes pre-treated with pronase, trypsin, alpha-chymotrypsin and thiol reducing agents as did anti-Gya and anti-Hy. In addition, similar to that found for anti-Gya and anti-Hy, anti Joa also showed reduced binding, as determined by haemagglutination and flow cytometric analysis, to erythrocytes from patients with paroxysmal nocturnal haemoglobinuria. Immune precipitates prepared from radio-iodinated antigen-positive red cells with anti-Joa, anti-Gya and anti-Hy gave similar results--a major component of M(r) 49,000-60,000 (the Gya/Hy-active glycoprotein) and a second component of M(r) 85,000-92,000 (this may be a dimer of the Gya/Hy-active glycoprotein, or a coprecipitated protein). These immune precipitates, when probed with both anti-Gya and anti-Hy under non-reducing conditions, gave a positive immunoblotting reaction to both the M(r) 49,000-60,000 and the M(r) 85,000-92,000 components. These results strongly suggest that the Joa antigen is expressed on the same glycoprotein that carries the Gya and Hy antigens.
通过血凝试验、流式细胞术分析、免疫沉淀和免疫印迹实验，研究了未分配的高发生率抗原Joa与Gya抗原组中的Gya和Hy抗原之间的表型关联。在血凝试验中，抗Joa与经链霉蛋白酶、胰蛋白酶、α-胰凝乳蛋白酶和硫醇还原剂预处理的红细胞产生的反应模式，与抗Gya和抗Hy相同。此外，与抗Gya和抗Hy的情况相似，抗Joa通过血凝和流式细胞术分析确定，与阵发性夜间血红蛋白尿患者的红细胞结合也减少。用抗Joa、抗Gya和抗Hy从放射性碘化抗原阳性红细胞制备的免疫沉淀物给出了相似的结果——一个主要成分的分子量为49,000 - 60,000（Gya/Hy活性糖蛋白），第二个成分的分子量为85,000 - 92,000（这可能是Gya/Hy活性糖蛋白的二聚体，或者是共沉淀蛋白）。这些免疫沉淀物在非还原条件下用抗Gya和抗Hy进行检测时，对分子量为49,000 - 60,000和85,000 - 92,000的成分均产生阳性免疫印迹反应。这些结果强烈表明，Joa抗原与携带Gya和Hy抗原的同一糖蛋白上表达。
