Martín Hernández A M, Camacho A, Prieto J, Menéndez del Campo A M, Tabarés E
Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Virus Res. 1995 Apr;36(1):67-75. doi: 10.1016/0168-1702(94)00098-w.
African swine fever virus induces the synthesis of thymidine kinase (TK) in BHK TK-negative cells as an immediate early protein. The TK gene is not essential for growth of ASFV in cell culture and a stable viral strain deficient in TK has been isolated (E70NTKp). The genetic lesion of this ASFV TK- strain was identified by TK gene nucleotide sequencing, showing a nucleotide deletion leading to a -1 frameshift and a nonsense codon residue downstream of the deletion. The availability of this viable ASFV variant deficient in TK activity allows the insertion of foreign genes in the ASFV genome for genetic and biochemical studies.
非洲猪瘟病毒在BHK TK阴性细胞中诱导胸苷激酶（TK）的合成，作为一种即刻早期蛋白。TK基因对于非洲猪瘟病毒在细胞培养中的生长并非必需，并且已分离出一种缺乏TK的稳定病毒株（E70NTKp）。通过TK基因核苷酸测序鉴定了这种非洲猪瘟病毒TK - 株的基因损伤，显示核苷酸缺失导致 - 1移码以及缺失下游的一个无义密码子残基。这种缺乏TK活性的可行非洲猪瘟病毒变体的存在允许将外源基因插入非洲猪瘟病毒基因组中进行遗传学和生物化学研究。
