Martin Hernandez A M, Tabares E
Departamento de Microbiologia, Facultad de Medicina, Universidad Autonoma de Madrid, Spain.
J Virol. 1991 Feb;65(2):1046-52. doi: 10.1128/JVI.65.2.1046-1052.1991.
The thymidine kinase (TK) gene of African swine fever virus (ASFV) was located within the viral genome by using two degenerate oligonucleotide probes derived from sequences of the vaccinia virus and cellular TK genes. The TK gene was mapped within a 0.72-kbp BglII-XhoI fragment (0.242 to 0.246 map units) derived from a 23.9-kbp SalI-B fragment of the ASFV genome. Identification of this region as the ASFV TK gene was confirmed by expression of TK in Escherichia coli and by the synthesis of active TK in a cell-free system programmed with RNA synthesized in vitro. The sequenced gene for TK includes an open reading frame of 588 nucleotides encoding a protein of 196 amino acids. The deduced amino acid sequence shows 32.4% identity with the TK of vaccinia virus.
通过使用源自痘苗病毒和细胞胸苷激酶（TK）基因序列的两个简并寡核苷酸探针，确定了非洲猪瘟病毒（ASFV）的胸苷激酶（TK）基因在病毒基因组中的位置。TK基因定位于从ASFV基因组23.9kbp的SalI - B片段衍生而来的0.72kbp BglII - XhoI片段（0.242至0.246个图距单位）内。通过在大肠杆菌中表达TK以及在用体外合成的RNA编程的无细胞系统中合成活性TK，证实了该区域为ASFV TK基因。TK的测序基因包含一个588个核苷酸的开放阅读框，编码一个196个氨基酸的蛋白质。推导的氨基酸序列与痘苗病毒的TK有32.4%的同源性。
