Lillrank S M, Oja S S, Saransaari P
Tampere Brain Reserach Center, Department of Biomedical Sciences, University of Tampere, Finland.
Acta Physiol Scand. 1995 Jan;153(1):33-41. doi: 10.1111/j.1748-1716.1995.tb09831.x.
The effects of handling and handling combined with phencyclidine (PCP) treatment on GABAergic neurotransmission were studied in Sprague-Dawley rats. The animal material consisted of handling-habituated (HH, for 11 d), acutely handled (naive, N), handling-habituated and PCP-treated (10 mg kg-1 i.p., HH + PCP) and acutely handled (naive) PCP-treated (N + PCP) and unhandled 'control' rats. The binding of [3H]GABA and [3H]flunitrazepam (FLU) was studied with membranes and the release of [3H]GABA with slices prepared from the striatum and frontal cortex. In the striatum the maximal binding capacity (Bmax) and the binding constant (KD) of [3H]GABA were the same in N and HH rats, but in the frontal cortex KD was lower in N rats. KD constants of [3H]FLU were significantly lower in both brain areas in N rats than in HH rats. After PCP treatment both Bmax and KD for [3H]FLU increased in these two brain areas in handling-habituated rats, whereas Bmax of [3H]GABA diminished. Neither handling nor PCP had any effect on [3H]GABA release from striatal and frontal cortical slices. Handling prior to killing thus affects differently the GABAergic parameters studied and modulates the PCP-induced effects.
在斯普拉格-道利大鼠中研究了处理以及处理与苯环利定（PCP）联合处理对γ-氨基丁酸能神经传递的影响。实验动物包括经处理适应的（HH，持续11天）、急性处理的（未经处理的，N）、经处理适应且接受PCP处理的（腹腔注射10 mg/kg，HH + PCP）、急性处理（未经处理的）且接受PCP处理的（N + PCP）以及未处理的“对照”大鼠。使用膜研究了[³H]γ-氨基丁酸（GABA）和[³H]氟硝西泮（FLU）的结合情况，并使用从纹状体和额叶皮质制备的切片研究了[³H]GABA的释放。在纹状体中，N组和HH组大鼠[³H]GABA的最大结合容量（Bmax）和结合常数（KD）相同，但在额叶皮质中，N组大鼠的KD较低。N组大鼠在这两个脑区中[³H]FLU的KD常数均显著低于HH组大鼠。在PCP处理后，经处理适应的大鼠这两个脑区中[³H]FLU的Bmax和KD均增加，而[³H]GABA的Bmax减小。处理和PCP对纹状体和额叶皮质切片中[³H]GABA的释放均无影响。因此，处死前的处理对所研究的γ-氨基丁酸能参数有不同影响，并调节PCP诱导的效应。
