Saldivar-Gonzalez A, Fernandez-Guasti A, Etgen A M
Division de Investigaciones en Neurociencias, Instituto Mexicano de Psiquiatria, CINVESTAV, IPN, D.F.
Brain Res. 1993 May 21;611(2):326-9. doi: 10.1016/0006-8993(93)90520-w.
The effect of male rat sexual behavior on the binding of [3H]-flunitrazepam (FNP) in frontal cortex, hypothalamus and hippocampus was measured. The groups included were: (A) control (no sexual experience) handled males; (B) male rats sacrificed 24 h after their fifth mating experience; (C) males sacrificed 48 h after their fifth mating experience; (D) male rats sacrificed after 5 intromissions during the sixth mating experience; and (E) males sacrificed immediately after the ejaculation during the sixth mating experience. Relative to handled controls, significant decreases in maximal [3H]-FNP binding (Bmax) were observed in frontal cortex and hippocampus of animals sacrificed after 5 intromissions and in frontal cortex of rats sacrificed 24 h after the last mating experience. Relative to males sacrificed after 5 intromissions, males sacrificed immediately after ejaculation exhibited a rapid increase in Bmax in frontal cortex and hippocampus. No between-group differences in Kd for [3H]-FNP were noted, and sexual experience had no effect on the Bmax of [3H]-FNP in the hypothalamus.
测定了雄性大鼠性行为对前额叶皮质、下丘脑和海马体中[³H] - 氟硝西泮（FNP）结合的影响。所设组包括：（A）对照（无性经验）的经处理雄性大鼠；（B）在第五次交配经验后24小时处死的雄性大鼠；（C）在第五次交配经验后48小时处死的雄性大鼠；（D）在第六次交配经验中经历5次插入动作后处死的雄性大鼠；以及（E）在第六次交配经验射精后立即处死的雄性大鼠。相对于经处理的对照，在经历5次插入动作后处死的动物的前额叶皮质和海马体中，以及在最后一次交配经验后24小时处死的大鼠的前额叶皮质中，观察到最大[³H] - FNP结合量（Bmax）显著降低。相对于在经历5次插入动作后处死的雄性大鼠，射精后立即处死的雄性大鼠在前额叶皮质和海马体中的Bmax迅速增加。未观察到[³H] - FNP的解离常数（Kd）在组间存在差异，并且性经验对下丘脑中[³H] - FNP的Bmax没有影响。
