双相情感障碍患者血小板中依赖环磷酸腺苷的内源性磷酸化异常。
Abnormalities of cAMP-dependent endogenous phosphorylation in platelets from patients with bipolar disorder.
作者信息
Perez J, Zanardi R, Mori S, Gasperini M, Smeraldi E, Racagni G
机构信息
Center for Neuropharmacology, Institute of Pharmacological Sciences, University of Milan, Italy.
出版信息
Am J Psychiatry. 1995 Aug;152(8):1204-6. doi: 10.1176/ajp.152.8.1204.
OBJECTIVE
The aim of the study was to assess cAMP-dependent endogenous phosphorylation in platelets from euthymic bipolar patients.
METHOD
Platelets from 10 drug-free euthymic patients with bipolar disorder were compared with those from 10 age- and sex-matched healthy subjects. Basal and cAMP-stimulated protein phosphorylation was examined in each group.
RESULTS
Endogenous phosphorylation in both the healthy volunteers and the bipolar patients was significantly stimulated by cAMP; the major polypeptides had apparent molecular weights of 38 and 22 kDa. The cAMP-stimulated 32P incorporation differed between the bipolar patients and the comparison subjects only in the 22-kDa band.
CONCLUSIONS
These data suggest a possible role of cAMP-dependent protein phosphorylation in the pathophysiology of bipolar disorder.
目的
本研究旨在评估心境正常的双相情感障碍患者血小板中依赖环磷酸腺苷（cAMP）的内源性磷酸化作用。
方法
将10例未服用药物且心境正常的双相情感障碍患者的血小板与10例年龄和性别匹配的健康受试者的血小板进行比较。检测每组的基础及cAMP刺激后的蛋白质磷酸化情况。
结果
cAMP可显著刺激健康志愿者和双相情感障碍患者的内源性磷酸化；主要多肽的表观分子量分别为38 kDa和22 kDa。双相情感障碍患者与对照组相比，cAMP刺激后的³²P掺入仅在22 kDa条带存在差异。
结论
这些数据表明依赖cAMP的蛋白质磷酸化在双相情感障碍的病理生理学中可能发挥作用。
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