Mitchell P B, Manji H K, Chen G, Jolkovsky L, Smith-Jackson E, Denicoff K, Schmidt M, Potter W Z
Experimental Therapeutics Branch, NIMH, Bethesda, Md., USA.
Am J Psychiatry. 1997 Feb;154(2):218-23. doi: 10.1176/ajp.154.2.218.
Previous investigations have suggested the involvement of signal-transducing guanine-nucleotide-binding proteins (G proteins) both in the mechanism of action of lithium and in the pathophysiology of bipolar affective disorder. To determine whether such G protein abnormalities are a trait phenomenon, the authors investigated the levels of G protein alpha subunits in platelets and lymphocytes of euthymic patients with bipolar affective disorder.
Selective antibodies were used to quantitate levels of G protein alpha subunits regulating adenylylcyclase activity (Gs alpha-both 45- and 52-kDa forms- and Gil-2 alpha) and those regulating phosphoinositide turnover (Gq/11 alpha) in both platelets and lymphocytes of 44 euthymic patients with bipolar affective disorder and 27 matched comparison subjects.
Levels of both Gs alpha 45 and Gs alpha 52 were higher in the platelets of the euthymic bipolar patients (both bipolar I and bipolar II) than in those of the comparison subjects.
These findings are consistent with previous reports of high Gs alpha levels in bipolar affective disorder and, furthermore, suggest that such levels may be a trait abnormality for this condition.
先前的研究表明，信号转导鸟嘌呤核苷酸结合蛋白（G蛋白）参与锂盐的作用机制以及双相情感障碍的病理生理学过程。为了确定这种G蛋白异常是否为一种特质现象，作者研究了双相情感障碍病情缓解期患者血小板和淋巴细胞中G蛋白α亚基的水平。
使用选择性抗体定量检测44例双相情感障碍病情缓解期患者和27例匹配的对照者血小板和淋巴细胞中调节腺苷酸环化酶活性的G蛋白α亚基（Gsα，包括45 kDa和52 kDa两种形式以及Gi1-2α）和调节磷脂酰肌醇代谢的G蛋白α亚基（Gq/11α）的水平。
双相情感障碍病情缓解期患者（包括双相I型和双相II型）血小板中Gsα 45和Gsα 52的水平均高于对照者。
这些发现与先前关于双相情感障碍中Gsα水平升高的报道一致，此外，还表明这种水平可能是该疾病的一种特质异常。
