Gallucci R M, Meadows G G
Department of Pharmaceutical Sciences, Washington State University, Pullman 99164-6510, USA.
Alcohol Clin Exp Res. 1995 Apr;19(2):402-9. doi: 10.1111/j.1530-0277.1995.tb01522.x.
Ethanol (20% w/v) given to female C57BL/6 mice in their drinking water reduces splenic natural killer (NK) cell cytolytic activity after 2, 4, and 10 weeks of consumption. This reduction is transient because the levels of NK cell cytotoxicity from ethanol-consuming mice are nearly equal to those of water-drinking mice after splenocytes were incubated in 1000 IU/ml of recombinant interleukin-2 (rIL2) for 16-18 hr. In this study, mice were given 20% w/v ethanol in the drinking water for 2 weeks. Splenic NK cells were enriched up to 88% by negative selection based on surface expression of NK1.1. Enriched NK cells were expanded in rIL2 for 6 days. Lymphokine-activated killer (LAK) cells from both ethanol-consuming and water-drinking mice were > 95% NK1.1+. LAK cell cytolytic activity was significantly lower against NK-insensitive P815 mastocytoma [6.67 +/- 2.18 vs. 17.21 +/- 1.8 lytic units (LUs), p < 0.01], moderately NK-sensitive B16 melanoma (25.3 +/- 6.6 vs. 66.2 +/- 14.2 LU, p < 0.05), and NK-sensitive YAC-1 lymphoma targets (80.5 +/- 34.7 vs. 177.0 +/- 43.6 LU, p < 0.005) in cells from ethanol-consuming mice compared with water-drinking controls. Ethanol consumption did not affect the morphology or phenotype of LAK cells with respect to surface expression of NK1.1, B220, CD3, CD25, CD11a, CD54, CD45RB, or class I major histocompatibility complex.
给雌性C57BL/6小鼠饮用含20%（重量/体积）乙醇的水，在饮用2周、4周和10周后，其脾脏自然杀伤（NK）细胞的细胞溶解活性降低。这种降低是短暂的，因为在将脾细胞在1000 IU/ml重组白细胞介素-2（rIL2）中孵育16 - 18小时后，饮用乙醇小鼠的NK细胞细胞毒性水平与饮用纯水小鼠的几乎相等。在本研究中，给小鼠饮用含20%（重量/体积）乙醇的水2周。基于NK1.1的表面表达，通过阴性选择将脾脏NK细胞富集至88%。富集的NK细胞在rIL2中扩增6天。来自饮用乙醇和饮用纯水小鼠的淋巴因子激活的杀伤（LAK）细胞中，> 95%为NK1.1 +。与饮用纯水的对照小鼠相比，饮用乙醇小鼠的LAK细胞对NK不敏感的P815肥大细胞瘤的细胞溶解活性显著降低[6.67 ± 2.18对17.21 ± 1.8溶解单位（LU），p < 0.01]，对中度NK敏感的B16黑色素瘤（25.3 ± 6.6对66.2 ± 14.2 LU，p < 0.05），以及对NK敏感的YAC - 1淋巴瘤靶标的细胞溶解活性也显著降低（80.5 ± 34.7对177.0 ± 43.6 LU，p < 0.005）。饮用乙醇并不影响LAK细胞在NK1.1、B220、CD3、CD25、CD11a、CD54、CD45RB或I类主要组织相容性复合体表面表达方面的形态或表型。
