Gallucci R M, Pfister L J, Meadows G G
Department of Pharmaceutical Sciences, Washington State University, Pullman 99164-6510.
Alcohol Clin Exp Res. 1994 Jun;18(3):625-31. doi: 10.1111/j.1530-0277.1994.tb00921.x.
Ethanol (20% w/v) given to female, C57BL/6 mice in their drinking water was previously shown to suppress natural killer (NK) cell cytolytic activity in a mixed splenocyte population. The present study was designed to examine the hypothesis that ethanol consumption independently results in inhibition of NK cell cytolytic activity. Mice were given 20% w/v ethanol in the drinking water for 2 weeks, and splenic NK cells were enriched up to 88% based on surface expression of NK1.1. Cytolytic activity of these freshly enriched NK cells from ethanol-consuming mice against YAC-1 lymphoma cells was inhibited an average of 41% relative to water-drinking controls. Cytolytic activity of enriched NK cells from ethanol-consuming mice was stimulated to levels equal to control water-drinking mice after 16- to 18-hr incubation in 1000 units/ml recombinant interleukin 2. These data indicate that in vitro cytolytic activity of NK cells from ethanol-consuming mice is inhibited in the absence of other modulatory leukocytes.
先前的研究表明，给雌性C57BL/6小鼠饮用含20%（重量/体积）乙醇的水，可抑制混合脾细胞群体中自然杀伤（NK）细胞的细胞溶解活性。本研究旨在检验乙醇摄入独立导致NK细胞细胞溶解活性受到抑制这一假说。给小鼠饮用含20%（重量/体积）乙醇的水2周，基于NK1.1的表面表达，脾NK细胞富集至88%。与饮用纯水的对照小鼠相比，这些刚富集的来自饮用乙醇小鼠的NK细胞对YAC-1淋巴瘤细胞的细胞溶解活性平均被抑制了41%。在1000单位/毫升重组白细胞介素2中孵育16至18小时后，来自饮用乙醇小鼠的富集NK细胞的细胞溶解活性被刺激至与饮用纯水的对照小鼠相当的水平。这些数据表明，在没有其他调节性白细胞的情况下，来自饮用乙醇小鼠的NK细胞的体外细胞溶解活性受到抑制。
