Dyck L E
Department of Psychiatry, College of Medicine, University of Saskatchewan, Saskatoon, Canada.
Alcohol Clin Exp Res. 1995 Apr;19(2):420-6. doi: 10.1111/j.1530-0277.1995.tb01525.x.
The types of aldehyde dehydrogenases (ALDH) present in human hair roots and in saliva were investigated. ALDH was detected by activity staining following separation of crude extracts by isoelectric focusing. Hair roots were found to express ALDH1, ALDH2, ALDH3, and ALDH4, whereas saliva expressed ALDH3. Two different patterns of ALDH3 were detected in hair roots collected from 42 donors, 40 expressed one pattern (variant I) and two another pattern (variant II) of activity staining. The variant I pattern of hair root ALDH3 changed with repetitive freezing and thawing of the sample, whereas the variant II pattern was stable. In contrast to hair root ALDH3, all patterns of ALDH3 activity in saliva were stable. The patterns of ALDH3 activity present in human hair roots that had been frozen and thawed twice matched those present in saliva collected from the same individual. Three polymorphisms of ALDH3 (variants I, II, and III) were detected in the 33 saliva samples analyzed. Variants I and II were inherited in each of three generations of a 10-member family.
对人发根和唾液中存在的醛脱氢酶（ALDH）类型进行了研究。通过等电聚焦分离粗提物后，采用活性染色法检测ALDH。发现发根表达ALDH1、ALDH2、ALDH3和ALDH4，而唾液表达ALDH3。在从42名供体采集的发根中检测到两种不同的ALDH3模式，40人表现出一种模式（变体I），另外两人表现出另一种活性染色模式（变体II）。发根ALDH3的变体I模式随样品反复冻融而变化，而变体II模式则稳定。与发根ALDH3不同，唾液中所有ALDH3活性模式都是稳定的。经过两次冻融的人发根中存在的ALDH3活性模式与从同一个体采集的唾液中的模式相匹配。在所分析的33份唾液样本中检测到ALDH3的三种多态性（变体I、II和III）。变体I和II在一个10口之家的三代人中均有遗传。
