Dyck L E
Department of Psychiatry, University of Saskatchewan, Saskatoon, Canada.
Hum Hered. 1993 Mar-Apr;43(2):116-20. doi: 10.1159/000154127.
Three methods were employed to assess whether human volunteers (Caucasian, Asian or Cree Indian) possessed the typical or atypical mitochondrial aldehyde dehydrogenase (ALDH2) isozyme. These methods were: (1) questioning individuals about facial flushing responses following alcohol consumption; (2) application of the ethanol skin patch test, and (3) direct analysis using isoelectric focusing and activity staining of ALDH activity in hair root samples. The results from the three methods were in good agreement and revealed that only the typical ALDH2 isozyme was expressed in Saskatchewan Cree Indians. In agreement with previous reports, the typical ALDH2 was expressed in the Caucasian group of subjects, while both the typical and atypical forms were expressed in the Asian subjects.
采用了三种方法来评估人类志愿者（白种人、亚洲人或克里印第安人）是否拥有典型或非典型的线粒体醛脱氢酶（ALDH2）同工酶。这些方法包括：（1）询问个体饮酒后脸红反应；（2）进行乙醇皮肤贴片试验；（3）使用等电聚焦和发根样本中ALDH活性的活性染色进行直接分析。这三种方法的结果高度一致，表明在萨斯喀彻温省的克里印第安人中仅表达典型的ALDH2同工酶。与先前的报道一致，典型的ALDH2在白种人受试者中表达，而在亚洲受试者中同时表达典型和非典型形式。
